02052nas a2200181   4500008004100000022001400041245015900055210006900214260009100283300002900374490000700403520124100410100002301651700002401674700002401698700002101722856012701743       2016                        eng d  a1932-620300aDynamics of colonization and expression of pathogenicity related genes in fusarium oxysporum f.sp ciceri during chickpea vascular wilt disease progression0 aDynamics of colonization and expression of pathogenicity related  a1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USAbPUBLIC LIBRARY SCIENCEcMAY  aArticle Number: e01564900 v113 a<p>Fusarium wilt caused by Fusarium oxysporum f.sp. ciceri (Foc) is a constant threat to chickpea productivity in several parts of the world. Understanding the molecular basis of chickpea-Foc interaction is necessary to improve chickpea resistance to Foc and thereby the productivity of chickpea. We transformed Foc race 2 using green fluorescent protein (GFP) gene and used it to characterize pathogen progression and colonization in wilt-susceptible (JG62) and wilt-resistant (Digvijay) chickpea cultivars using confocal microscopy. We also employed quantitative PCR (qPCR) to estimate the pathogen load and progression across various tissues of both the chickpea cultivars during the course of the disease. Additionally, the expression of several candidate pathogen virulence genes was analyzed using quantitative reverse transcriptase PCR (qRT-PCR), which showed their characteristic expression in wilt-susceptible and resistant chickpea cultivars. Our results suggest that the pathogen colonizes the susceptible cultivar defeating its defense; however, albeit its entry in the resistant plant, further proliferation is severely restricted providing an evidence of efficient defense mechanism in the resistant chickpea cultivar.</p>1 aUpasani, Medha, L.1 aGurjar, Gayatri, S.1 aKadoo, Narendra, Y.1 aGupta, Vidya, S.  uhttps://library.ncl.res.in/content/dynamics-colonization-and-expression-pathogenicity-related-genes-fusarium-oxysporum-fsp