02134nas a2200265   4500008004100000022001400041245013000055210006900185260007900254300001200333490000800345520113900353653002701492653001401519653001601533653002601549653003201575653001601607100002501623700002801648700002301676700002001699700002001719856012901739       2005                        eng d  a1096-495900aUnraveling biochemical properties of cockroach (Periplaneta americana) proteinases with a gel X-ray film contact print method0 aUnraveling biochemical properties of cockroach Periplaneta ameri  a360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USAbELSEVIER SCIENCE INCcJUL  a261-2660 v1413 a<p>Eleven proteinase activity bands were detected in American cockroach (Periplaneta americana) gut. These were partially purified and characterized using a gel X-ray film contact print method. Cockroach gut proteinases (CGPs) show activity over a broad range of pH with maximum activity between pH 6 and 10, and optimal activity at 50-70 degrees C. CGPs were partially purified by preparative gel electrophoresis and analyzed using synthetic substrates and inhibitors. Four of the proteases exhibited chymotrypsin-like (C1 to C4) activity and seven trypsin-like (T I to T7) activity. Accuracy of the gel X-ray film contact print method is confirmed by including bovine chymotrypsin in CGP analysis. Inhibition of CGPs with different plant proteinaceous proteinase inhibitors allowed identification of potential CGP inhibitors. Our results imply that presence of several CGP activity bands, and their stability and activity over a broad pH and temperature range might contribute to adaptation of P americana to extreme environmental conditions and the polyphagous nature of the species. (c) 2005 Elsevier Inc. All rights reserved.</p>10aactivity visualization10acockroach10agut enzymes10aPeriplaneta americana10aplant proteinase inhibitors10aProteinases1 aHivrale, Vandana, K.1 aChougule, Nanasaheb, P.1 aChhabda, Pavan, J.1 aGiri, Ashok, P.1 aKachole, M., S.  uhttps://library.ncl.res.in/content/unraveling-biochemical-properties-cockroach-periplaneta-americana-proteinases-gel-x-ray-0