01830nas a2200241   4500008004100000022001400041245008600055210006900141260010200210300001200312490000700324520094700331653002001278653001601298653003001314653001001344653002001354100003301374700002401407700001901431700001801450856012001468       2006                        eng d  a1054-547600aRepetitive somatic embryogenesis and plant regeneration in Garcinia indica Choiss0 aRepetitive somatic embryogenesis and plant regeneration in Garci  aC/O PUBLISHING DIVISION, NOSWORTHY WAY, WALLINGFORD OX10 8DE, OXON, ENGLANDbCABI PUBLISHINGcMAY  a256-2610 v423 a<p>Immature seeds of Garcinia indica Choiss. were excised from immature fruits and cultured on Lloyd and McCown (1980), woody plant medium (WPM) with different combinations of auxins and cytokinins. Somatic embryos were obtained on the media supplemented with 6-benzylaminopurine (BA; 2.2-22.1 mu M) alone or in combination with alpha-naphthalene acetic acid (NAA; 2.6 mu M) with 80% frequency within a period of 2-3 wk. Subculture of embryos on medium containing BA (16.0 mu M) supplemented with indole-3-acetic acid (IAA; 2.8-5.7 mu M) and/or kinetin (4.6 mu M) gave rise to clusters of secondary somatic embryos along with maturation of primary embryos. In subsequent subculture on hormone-free half-strength WPM, the embryo clusters germinated with an increase in the number of secondary somatic embryos. About 70% of somatic embryos germinated into complete plantlets, which were successfully established under greenhouse conditions.</p>10aGarcinia indica10aguttifereae10asecondary somatic embryos10aseeds10asomatic embryos1 aThengane, Shubhada, Ratnakar1 aDeodhar, Swapna, R.1 aBhosle, S., V.1 aRawal, S., K.  uhttps://library.ncl.res.in/content/repetitive-somatic-embryogenesis-and-plant-regeneration-garcinia-indica-choiss-0