01800nas a2200229   4500008004100000022001400041245014700055210006900202260005700271300001200328490000700340520091600347653002501263653002501288653002101313653001801334653002201352100002601374700002201400700001601422856013201438       2006                        eng d  a0959-399300aRole of glucose in production and repression of polygalacturonase and pectate lyase from phytopathogenic fungus Fusarium moniliforme NCIM 12760 aRole of glucose in production and repression of polygalacturonas  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcSEP  a893-8990 v223 a<p>A mangrove fungal isolate Fusarium moniliforme NCIM 1276 was found to produce the maximum extracellular endopolygalacturonase and pectate lyase activities in liquid medium containing 1% citrus pectin and 0.2% glucose at pH 5 and pH 8, respectively. The enzyme secretion started after 10 h, when the spores were completely germinated, and maximum production occurred after 72 h in the extracellular medium. The production of polygalacturonase was enhanced by 0.2% glucose and completely repressed by 1% glucose. Pectate lyase secretion was also induced by a similar concentration of glucose in the extracellular medium and significantly decreased at 1% glucose. Quantification of extracellular and intracellular polygalacturonase and pectate lyase protein concentrations by ELISA and immunocytolocalization data suggested that glucose may control the expression of both enzymes at a transcriptional level.</p>10acatabolic repression10aFusarium moniliforme10ainducible enzyme10apectate lyase10apolygalacturonase1 aNiture, Suryakant, K.1 aKumar, Ameeta, R.1 aPant, Aditi  uhttps://library.ncl.res.in/content/role-glucose-production-and-repression-polygalacturonase-and-pectate-lyase-phytopathogenic-0