01680nas a2200229   4500008004100000022001400041245008200055210006900137260008700206300001400293490000700307520086000314653002001174653001601194653001001210653002001220100003301240700002401273700001901297700001801316856011601334       2006                        eng d  a0011-389100aDirect somatic embryogenesis and plant regeneration in Garcinia indica Choiss0 aDirect somatic embryogenesis and plant regeneration in Garcinia   aC V RAMAN AVENUE, PO BOX 8005, BANGALORE 560 080, INDIAbCURRENT SCIENCE ASSNcOCT  a1074-10780 v913 a<p>Direct somatic embryogenesis without an intervening callus phase was induced from immature seeds of Gar- cinia indica Choiss. Woody plant medium supplemented with 6-benzyl amino purine (BAP) (4.44-22.19 mu m) alone or in combination with a-naphthaleneacetic acid (2.69 mu m) produces somatic embryos within a period of 2-3 weeks with 80% frequency. Embryo induction was observed all over the explant surface. Origin of the embryo was confirmed histologically from sub-epidermal layer of the seed. Maturation of these embryos was achieved after 12 weeks of culture on a medium containing BAP (16.08 mu m) in combination with indole-3-acetic acid (2.85-5.71 mu m) and/or kinetin (4.65 mu m). About 75% of the regenerated somatic embryos germinated into complete plantlets. The plantlets were acclimatized successfully with 92% survival in greenhouse.</p>10aGarcinia indica10aguttifereae10aseeds10asomatic embryos1 aThengane, Shubhada, Ratnakar1 aDeodhar, Swapna, R.1 aBhosle, S., V.1 aRawal, S., K.  uhttps://library.ncl.res.in/content/direct-somatic-embryogenesis-and-plant-regeneration-garcinia-indica-choiss-0