02294nas a2200241   4500008004100000022001400041245009300055210006900148260006700217300001000284490000700294520147800301653001701779653002001796653001401816653001101830653001201841653001401853100001601867700001701883700002601900856012601926       2007                        eng d  a1521-654300aChemical, thermal and pH-induced equilibrium unfolding studies of fusarium solani lectin0 aChemical thermal and pHinduced equilibrium unfolding studies of   a111 RIVER ST, HOBOKEN 07030-5774, NJ USAbWILEY-BLACKWELLcDEC  a34-430 v593 a<p>The effect of urea, guanidine thiocyanate, temperature and pH was studied on the conformational stability of Fusarium solani lectin. Equilibrium unfolding with chemical denaturants showed that the lectin was least stable at pH 12 and maximally stable at pH 8.0 near its pI ( 8.7). Guanidine thiocyanate ( the concentration of denaturant at which the protein is half folded, D-1/2 = 0.49 M at pH 12) was found to be an eight times stronger denaturant than urea (D-1/2 = 3.88 M at pH 12). The unfolding curves obtained with fluorescence and CD measurements showed good agreement indicating a monophasic nature of unfolding and excluded the possibility of formation of any stable intermediate. The effect of pH on the lectin was found to be unusual as at acidic pH, the lectin showed a flexible tertiary structure with pronounced secondary structure, and retained its hemagglutinating activity. On the other hand, the lectin did not show any loss of conformation or activity upto 70 degrees C for 15 min. Moreover, thermal denaturation did not result in the aggregation or precipitation of the protein even at high temperatures. Thermal denaturation was also carried out in the presence of a low concentration of guanidine thiocyanate. Change in the enthalpy of transition (Delta H-m) varied linearly with transition temperature (T-m), which indicated that the heat capacity (Delta C-p =3.95 kJ (.) mol(-1) (.) K-1) of the lectin remained constant during the unfolding.</p>10aDenaturation10aFusarium solani10aguanidine10aLectin10athermal10aUnfolding1 aKhan, Feroz1 aAhmad, Absar1 aKhan, Mohammad, Islam  uhttps://library.ncl.res.in/content/chemical-thermal-and-ph-induced-equilibrium-unfolding-studies-fusarium-solani-lectin-0