01616nas a2200181   4500008004100000022001400041245014000055210006900195260007200264300000900336490000700345520086300352100001801215700002401233700002201257700002601279856012901305       2007                        eng d  a0305-104800aVersatile method for the preparation of conjugates of peptides with DNA/PNA/analog by employing chemo-selective click reaction in water0 aVersatile method for the preparation of conjugates of peptides w  aGREAT CLARENDON ST, OXFORD OX2 6DP, ENGLANDbOXFORD UNIV PRESScDEC  ae1390 v353 a<p>The specific 1,3 dipolar Hisgen cycloaddition reaction known as `click-reaction' between azide and alkyne groups is employed for the synthesis of peptideoligonucleotide conjugates. The peptide nucleic acids (PNA)/DNA and peptides may be appended either by azide or alkyne groups. The cycloaddition reaction between the azide and alkyne appended substrates allows the synthesis of the desired conjugates in high purity and yields irrespective of the sequence and functional groups on either of the two substrates. The versatile approach could also be employed to generate the conjugates of peptides with thioacetamido nucleic acid (TANA) analog. The click reaction is catalyzed by Cu (I) in either water or in organic medium. In water, similar to 3-fold excess of the peptide-alkyne/azide drives the reaction to completion in 2 h with no side products.</p>1 aGogoi, Khirud1 aMane, Meenakshi, V.1 aKunte, Sunita, S.1 aKumar, Vaijayanti, A.  uhttps://library.ncl.res.in/content/versatile-method-preparation-conjugates-peptides-dnapnaanalog-employing-chemo-selective-0