01831nas a2200229   4500008004100000022001400041245006500055210006500120260009500185300001400280490000600294520104900300653001701349653001701366653002301383100002101406700002101427700001401448700002101462700001901483856009901502       2007                        eng d  a1533-488000aBacterial enzyme mediated biosynthesis of gold nanoparticles0 aBacterial enzyme mediated biosynthesis of gold nanoparticles  a25650 NORTH LEWIS WAY, STEVENSON RANCH, CA 91381-1439 USAbAMER SCIENTIFIC PUBLISHERScDEC  a4369-43770 v73 a<p>Development of synthesis methods for anisotropic metal nanoparticles is of considerable interest due to their remarkable optoelectronic properties. Various shapes ranging from rods to cubes to tetrapods and prisms may be obtained by chemical methods. Here we show that anisotropic gold nanoparticles can be synthesized biologically by the bacterium Actinobacter spp. when challenged with gold chloride in the presence of Bovine serum albumin (BSA). We also observed that synthesis of gold nanoparticles occur with simultaneous induction of the protease enzyme secreted by the bacterium in the presence of BSA. The presence of BSA helps to enhance the rate of gold nanoparticles biosynthesis and may also impart some shape control. Controlling simple experimental conditions like incubation temperature and presence or absence of oxygen have drastic effect on the reaction rate and the morphology of the particles. Various assay experiments show that the presence of enzyme protease can act as a reducing as well as shape directing agent.</p>10aBiosynthesis10abovine serum10agold nanoparticles1 aBharde, Atul, A.1 aKulkarni, Aarohi1 aRao, Mala1 aPrabhune, Asmita1 aSastry, Murali  uhttps://library.ncl.res.in/content/bacterial-enzyme-mediated-biosynthesis-gold-nanoparticles-0