02042nas a2200229   4500008004100000022001400041245016000055210006900215260009700284300001000381490000800391520111700399653002501516653001701541653001801558653002201576100002601598700002201624700002301646700001601669856012701685       2008                        eng d  a0944-501300aInactivation of polygalacturonase and pectate lyase produced by pH tolerant fungus Fusarium moniliforme NCIM 1276 in a liquid medium and in the host tissue0 aInactivation of polygalacturonase and pectate lyase produced by   aOFFICE JENA, P O BOX 100537, 07705 JENA, GERMANYbELSEVIER GMBH, URBAN & FISCHER VERLAGcJAN  a51-620 v1633 a<p>Fusarium moniliforme NCIM 1276 produced pH dependent an extracellular potygalacturonase (PG) and pectate lyase (PL) at pH 5 and pH 8, respectively. In the extracellular medium about 20.3% PG and 54% of PL protein concentrations were present in the active state at pH 5 and pH 8, respectively, whereas in intracelluarly, more than 86% of both protein contents remained in the active state at all pH tested. We found two possible reasons, end-product inhibition and effect of environmental. pH on conformation of the proteins after their release into the medium. Additionally, in infected tomato and cauliflower plants, the fungus secreted similar proteins which were located near to the epidermal and vascular regions of the hypocotyts. In infected tissues, between 26.9% and to 41.5% of PG and only 0.84% - 13.4% of PL protein concentrations were present in active state. Thus, the medium/cell sap pH and concentrations of substrate/end products seem to play an important role in fungal invasion during plant pathogenesis are discussed with current literature. (c) 2006 Elsevier GmbH. All rights reserved.</p>10aFusarium moniliforme10apathogenesis10apectate lyase10apolygatacturonase1 aNiture, Suryakant, K.1 aKumar, Ameeta, R.1 aParab, Pradeep, B.1 aPant, Aditi  uhttps://library.ncl.res.in/content/inactivation-polygalacturonase-and-pectate-lyase-produced-ph-tolerant-fungus-fusarium-0