01611nas a2200229   4500008004100000022001400041245007600055210006900131260008900200300001200289490000700301520081500308653002001123653001601143653001801159653001001177653001101187100002401198700002101222700003301243856010501276       2008                        eng d  a0019-518900aDe novo shoot regeneration from root cultures of garcinia indica choiss0 aDe novo shoot regeneration from root cultures of garcinia indica  aDR K S KRISHNAN MARG, NEW DELHI 110 012, INDIAbNATL INST SCIENCE COMMUNICATIONcJUN  a482-4860 v463 a<p>Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medium supplemented with BAP (0.44-2.22 mu M) showed production of de novo shoots. Roots attached to mother plant showed more number of shoots, while excised root segments produced lesser shoots. Shoots (0.5-0.8 cm) were transferred to elongation medium consisting of Woody Plant Medium (WPM) supplemented with BAP (4.44-22.69 mu M), IAA (5.71 mu M and kinetin (4.65 mu M). It was observed that shoot length increased to 1-2 cm. WPM medium supplemented with NAA (2.69-10.74 mu M) and IBA (4.90 mu M) induced rooting within 20-25 days. Using the present protocol, 20-25 plantlets could be regenerated from single root explant within 3 to 4 months. The protocol has potential for large scale production of elite plants.</p>10aGarcinia indica10aguttifereae10aorganogenesis10aroots10ashoots1 aDeodhar, Swapna, R.1 aThengane, R., J.1 aThengane, Shubhada, Ratnakar  uhttps://library.ncl.res.in/content/de-novo-shoot-regeneration-root-cultures-garcinia-indica-choiss-0