01845nas a2200229   4500008004100000022001400041245013700055210006900192260009900261300001400360490000700374520092000381653002601301653002601327653002801353100002801381700002401409700001901433700002101452700002101473856012101494       2008                        eng d  a0960-852400aImmobilization of Candida rugosa lipase on poly(allyl glycidyl ether-co-thylene glycol dimethacrylate) macroporous polymer particles0 aImmobilization of Candida rugosa lipase on polyallyl glycidyl et  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcJUN  a3623-36290 v993 a<p>Macroporous polymer particles containing surface epoxy groups were synthesized for immobilization of Candida rugosa lipase (CRL). The effect of incorporation of two different sets of monomers [allyl glycidyl ether (AGE) and glycidyl methacrylate (GMA)] and the effect of crosslinking density on immobilization of lipase were studied. AGE-co-EGDM polymers gave higher binding and expression of lipase than GMA-co-EGDM polymers. Optimization of immobilization parameters was done with respect to immobilization time and enzyme loading. Amongst AGE-co-EGDM polymer series, AGE-150 polymer found to give maximum lipase activity yield and therefore evaluated for temperature, pH and storage stability. Under optimum conditions, AGE-150 polymer gave 78.40% of activity yield. Immobilized lipase on AGE-150 showed a broader pH, higher temperature and excellent storage stability. (C) 2007 Published by Elsevier Ltd.</p>10aCandida rugosa lipase10aEnzyme immobilization10aEpoxy-activated support1 aVaidya, Bhalchandra, K.1 aIngavle, Ganesh, C.1 aPonrathnam, S.1 aKulkarni, B., D.1 aNene, Sanjay, N.  uhttps://library.ncl.res.in/content/immobilization-candida-rugosa-lipase-polyallyl-glycidyl-ether-co-thylene-glycol-0