01458nas a2200229   4500008004100000022001400041245007600055210006900131260010600200300001400306490000700320520064200327653001400969653002000983653001801003653002301021100001701044700001601061700002101077700002601098856010401124       2008                        eng d  a0960-894X00aSerine protease inhibitor from hemolymph of green mussel, perna viridis0 aSerine protease inhibitor from hemolymph of green mussel perna v  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcJUL  a3963-39670 v183 a<p>Bioactivity guided fractions of cell-free hemolymph of bacterially challenged marine mussel, Perna viridis led to the isolation of a novel quaternary alkaloid 1, which was identified by its spectral data. The isolated molecule 1 has been found to be a potent serine protease inhibitor ( SPI) showing IC(50) and K(i) values of 102.5 and 97.1-104.68 mu M, respectively. The E(t)/K(i) value of SPI is 6.3, whereas E(t)/K(m) value is 1.04. The Van't Hoff analysis showed that the value of K(i) decreases with increase in temperature, and the binding of the inhibitor is entropically driven. (c) 2008 Elsevier Ltd. All rights reserved.</p>10ahemolymph10aNatural product10aPerna viridis10aprotease inhibitor1 aKhan, M., S.1 aGoswami, U.1 aRojatkar, S., R.1 aKhan, Mohammad, Islam  uhttps://library.ncl.res.in/content/serine-protease-inhibitor-hemolymph-green-mussel-perna-viridis-0