02292nas a2200217   4500008004100000022001400041245006300055210006200118260012500180300001100305490000800316520150400324100002001828700001801848700001901866700001701885700002501902700002701927700002301954856009701977       2008                        eng d  a0021-897900aZinc oxide nanoparticles as novel alpha-amylase inhibitors0 aZinc oxide nanoparticles as novel alphaamylase inhibitors  aCIRCULATION & FULFILLMENT DIV, 2 HUNTINGTON QUADRANGLE, STE 1 N O 1, MELVILLE, NY 11747-4501 USAbAMER INST PHYSICScNOV  a0949070 v1043 a<p>Amylase inhibitors, also known as starch blockers, contain substances that prevent dietary starches from being absorbed by the body via inhibiting breakdown of complex sugars to simpler ones. In this sense, these materials are projected as having potential applications in diabetes control. In this context, we report on zinc oxide nanoparticles as possible alpha-amylase inhibitors. Zinc oxide nanoparticles have been synthesized using soft-chemistry approach and 1-thioglycerol was used as a surfactant to yield polycrystalline nanoparticles of size similar to 18 nm, stabilized in wurtzite structure. Conjugation study and structural characterization have been done using x-ray diffraction technique, Fourier transform infrared spectroscopy, UV-visible spectroscopy, and transmission electron microscopy. Cytotoxicity studies on human fibrosarcoma (HT-1080) and skin carcinoma (A-431) cell lines as well as mouse primary fibroblast cells demonstrate that up to a dose of 20 mu g/ml, ZnO nanoparticles are nontoxic to the cells. We report for the first time the alpha-amylase inhibitory activity of ZnO nanoparticles wherein an optimum dose of 20 mu g/ml was sufficient to exhibit 49% glucose inhibition at neutral pH and 35 degrees C temperature. This inhibitory activity was similar to that obtained with acarbose (a standard alpha-amylase inhibitor), thereby projecting ZnO nanoparticles as novel alpha-amylase inhibitors. (C) 2008 American Institute of Physics. [DOI: 10.1063/1.3009317]</p>1 aDhobale, Sandip1 aThite, Trupti1 aLaware, S., L.1 aRode, C., V.1 aKoppikar, Soumya, J.1 aGhanekar, Ruchika-Kaul1 aKale, Sangeeta, N.  uhttps://library.ncl.res.in/content/zinc-oxide-nanoparticles-novel-alpha-amylase-inhibitors-0