02521nas a2200277   4500008004100000022001400041245012800055210006900183260007300252300001200325490000700337520149500344653003001839653002601869653002401895653001901919100002001938700002801958700001401986700001702000700002602017700002102043700003102064700002002095856012802115       2008                        eng d  a0924-857900aPotential plasmid-curing agent, 8-epidiosbulbin E acetate, from dioscorea bulbifera L. against multidrug-resistant bacteria0 aPotential plasmidcuring agent 8epidiosbulbin E acetate from dios  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcNOV  a405-4100 v323 a<p>Bioassay-guided fractionation of an aqueous methanolic extract of Dioscorea bulbifera L. bulbs was performed using organic solvents. A novel plasmid-curing compound was identified as 8-epidiosbulbin E acetate (EEA) (norditerpene) on the basis of modern spectroscopic analysis and X-ray crystallography. EEA exhibited broad-spectrum plasmid-curing activity against multidrug-resistant (MDR) bacteria, including vancomycin-resistant enterococci. EEA cured antibiotic resistance plasmids (R-plasmids) from clinical isolates of Enterococcus faecalis, Escherichia coli, Shigella sonnei and Pseudomonas aeruginosa with 12-48% curing efficiency. The reference plasmids of Bacillus subtilis (pUB110), E. coli (RP4), P. aeruginosa (RIP64) and Salmonella typhi (R136) were cured with efficiency ranging from 16% to 64%. EEA-mediated R-plasmid curing decreased the minimal inhibitory concentration of antibiotics against MDR bacteria, thus making antibiotic treatment more effective. The antibiotic resistance pattern revealed that the compound was effective in the reversal of bacterial resistance to various antibiotics. In addition, the compound did not show any cytotoxicity against a broad range of human cancer cell lines, namely MCF-7 (breast cancer), SiHa (cervical cancer) and A431 (epidermal carcinoma), and hence has the potential to be used as a lead compound for drug discovery programmes. (C) 2008 Elsevier B. V. and the International Society of Chemotherapy. All rights reserved.</p>10a8-Epidiosbulbin E acetate10aAntibiotic resistance10aDioscorea bulbifera10aPlasmid curing1 aShriram, Varsha1 aJahagirdar, Sheetal, S.1 aLatha, C.1 aKumar, Vinay1 aPuranik, Vedavati, G.1 aRojatkar, Supada1 aDhakephalkar, Prashant, K.1 aShitole, M., G.  uhttps://library.ncl.res.in/content/potential-plasmid-curing-agent-8-epidiosbulbin-e-acetate-dioscorea-bulbifera-l-against-0