02078nas a2200253   4500008004100000022001400041245019000055210006900245260007900314300001200393490000800405520106000413653002801473653002501501653002401526653002101550653002701571100001601598700001801614700001901632700002101651700002001672856013201692       2009                        eng d  a0273-228900aEffect of cultural conditions and media constituents on production of penicillin V acylase and CTAB treatment to enhance whole-cell enzyme activity of rhodotorula aurantiaca (NCIM 3425)0 aEffect of cultural conditions and media constituents on producti  a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcJUN  a463-4720 v1573 a<p>Penicillin V acylase (PVA) is a pharmaceutically important enzyme as it plays a vital role in the manufacture of semi-synthetic beta-lactam antibiotics. Rhodotorula aurantiaca (NCIM 3425) produced high levels of intracellular penicillin V acylase after 18 h at pH 8.0 and temperature 27 degrees C. Fructose was the best carbon source for PVA production, whereas tryptone was the best nitrogen source to produce the enzyme up to 170 and 1,088 IU/l of culture, respectively. Additionally, the cell-bound PVA activity was enhanced on treatment with cationic detergent. Whole-cell activity was found to be doubled (204%) on treatment of 0.01 g dry weight of cells with 50 mu g/ml solution of N-cetyl-N,N,N-trimethylammoniumbromide at pH 8.0 for 1 h at room temperature. Atomic force microscopy images of permeabilized cells show perturbation in the cell wall and offer first-ever visual illustration of surface structure modifications that occur during permeabilization of R. aurantiaca cells leading to enhancement in activity of intracellular enzyme.</p>10aAtomic force microscopy10aIntracellular enzyme10aPenicillin Vacylase10aPermeabilization10aRhodotorula aurantiaca1 aKumar, Atul1 aSingh, Sanjay1 aPoddar, Pankaj1 aPrabhune, Asmita1 aPundle, Archana  uhttps://library.ncl.res.in/content/effect-cultural-conditions-and-media-constituents-production-penicillin-v-acylase-and-ctab-0