01712nas a2200157   4500008004100000022001400041245013400055210006900189260011000258300001200368490000600380520100300386100002001389700001901409856012601428       2009                        eng d  a2040-336400aProbing differential Ag+-nucleobase interactions with isothermal titration calorimetry (ITC): towards patterned DNA metallization0 aProbing differential Agnucleobase interactions with isothermal t  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcOCT  a122-1270 v13 a<p>DNA has been Successfully used as a scaffold for the fabrication of metallic nanowires, primarily based oil the electrostatic complexation and reduction of the metal cations on the negatively charged sugar-phosphate backbone. Here. we probe the differential binding affinities of nucleobases for silver ions using sensitive isothermal titration calorimetry (ITC) measurements of the reaction enthalpies, which go in order: C &gt; G &gt; A &gt;= T. Using the disparity between the interaction of cytosine (strong binding) and thymine (weak binding) with silver ions, we have successfully generated silver nanoparticle doublets and triplets on custom-made oligonucleotides, C-30-T-40-C-30 and C-20-T-20-C-20-T-20-C-20, respectively. Thus, it new and simple method of generating metallized DNA wires is presented, based entirely on the nucleotide sequence of DNA. The concept could be extended to other cations and complex DNA sequences in order to achieve intricately patterned DNA constructs.</p>1 aShukla, Sourabh1 aSastry, Murali  uhttps://library.ncl.res.in/content/probing-differential-ag-nucleobase-interactions-isothermal-titration-calorimetry-itc-0