01704nas a2200181   4500008004100000022001400041245007000055210006700125260007700192300001400269490000700283520100900290100003201299700003001331700002701361700003001388856010401418       2010                        eng d  a1616-301X00aNanogold-Loaded sharp-edged carbon bullets as plant-gene carriers0 aNanogoldLoaded sharpedged carbon bullets as plantgene carriers  aPO BOX 10 11 61, D-69451 WEINHEIM, GERMANYbWILEY-V C H VERLAG GMBHcAUG  a2416-24230 v203 a<p>The higher DNA delivery efficiency into plants by gold nanoparticles embedded in sharp carbonaceous carriers is demonstrated. These nanogold-embedded carbon matrices are prepared by heat treatment of biogenic intracellular gold nanoparticles. The DNA-delivery efficiency is tested on a model plant, Nicotiana tabacum, and is further extended to the monocot, Oryza sativa, and a hard dicot tree species, Leucaena leucocephala. These materials reveal good dispersion of the transport material, producing a greater number of GUS foci per unit area. The added advantages of the composite carrier are the lower plasmid and gold requirements. Plant-cell damage with the carbon-supported particles is very minimal and can be, gauged from the increased plant regeneration and transformation efficiency compared with that of the commercial micrometer-sized gold particles. This is ascribed to the sharp edges that the carbon supports possess, which lead to better piercing capabilities with minimum damage.</p>1 aVijayakumar, Periyasamy, S.1 aAbhilash, Othalathara, U.1 aKhan, Bashir, Mohammad1 aPrasad, Bhagavatula L. V.  uhttps://library.ncl.res.in/content/nanogold-loaded-sharp-edged-carbon-bullets-plant-gene-carriers-0