02101nas a2200253   4500008004100000022001400041245015000055210006900205260009900274300001400373490000800387520111000395653001901505653003601524653004101560653001201601653001101613100001801624700002301642700002101665700002001686700001401706856012701720       2011                        eng d  a0960-852400aMicrobial production of xylitol from D-xylose and sugarcane bagasse hemicellulose using newly isolated thermotolerant yeast Debaryomyces hansenii0 aMicrobial production of xylitol from Dxylose and sugarcane bagas  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcFEB  a3304-33080 v1023 a<p>A thermotolerant yeast capable of fermenting xylose to xylitol at 40 C was isolated and identified as a strain of Debaryomyces hansenii by ITS sequencing. This paper reports the production of xylitol from D-xylose and sugarcane bagasse hemicellulose by free and Ca-alginate immobilized cells of D. hansenii. The efficiency of free and immobilized cells were compared for xylitol production from D-xylose and hemicellulose in batch culture at 40 degrees C. The maximum xylitol produced by free cells was 68.6 g/L from 100 g/L of xylose, with a yield of 0.76 g/g and volumetric productivity 0.44 g/L/h. The yield of xylitol and volumetric productivity were 0.69 g/g and 0.28 g/L/h respectively from hemicellulosic hydrolysate of sugarcane bagasse after detoxification with activated charcoal and ion exchange resins. The Ca-alginate immobilized D. hansenii cells produced 73.8 g of xylitol from 100 g/L of xylose with a yield of 0.82 g/g and volumetric productivity of 0.46 g/L/h and were reused for five batches with steady bioconversion rates and yields. (C) 2010 Elsevier Ltd. All rights reserved.</p>10aImmobilization10aSugarcane bagasse hemicellulose10aThermotolerant Debaryomyces hansenii10axylitol10aXylose1 aPrakash, Gyan1 aVarma, Anjanikumar1 aPrabhune, Asmita1 aShouche, Yogesh1 aRao, Mala  uhttps://library.ncl.res.in/content/microbial-production-xylitol-d-xylose-and-sugarcane-bagasse-hemicellulose-using-newly-0