02255nas a2200265   4500008004100000022001400041245013400055210006900189260009500258300001200353490000800365520121200373653002101585653002401606653003501630653001901665653002301684100002501707700002401732700002801756700002501784700002601809700003001835856012401865       2011                        eng d  a0003-269700aCarbon nanotube-modified sodium dodecyl sulfate-polyacrylamide gel electrophoresis for molecular weight determination of proteins0 aCarbon nanotubemodified sodium dodecyl sulfatepolyacrylamide gel  a525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USAbACADEMIC PRESS INC ELSEVIER SCIENCEcFEB  a230-2350 v4093 a<p>The effect of incorporating carbon nanotubes (CNTs) in the gel matrix on the electrophoretic mobility of proteins based on their molecular weight differences was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). More specifically, a reduction in standard deviation in the molecular weight calibration plots by 55% in the case of multiwalled carbon nanotubes (MWCNTs) and by 34% in the case of single-walled carbon nanotubes (SWCNTs) compared with that of pristine polyacrylamide gels was achieved after incorporating an insignificant amount of functionalized CNTs into the gel matrix. A mechanism based on a more uniform pore size distribution in CNT modified polyacrylamide gel matrix is proposed. Furthermore, the impact of SWCNTs and MWCNTs on the mobility of proteins in different molecular weight regimes at a given acrylamide concentration offers a tunable gel matrix in terms of the selection of molecular weight ranges of proteins. The robustness and excellent reproducibility of the CNT-PAGE protocol are expected to have a significant impact on the molecular weight determination of newly isolated proteins. (C) 2010 Elsevier Inc. All rights reserved.</p>10aCarbon nanotubes10aGel electrophoresis10aMolecular weight determination10aPolyacrylamide10aProtein separation1 aParthasarathy, Meera1 aDebgupta, Joyashish1 aKakade, Bhalchandra, A.1 aAnsary, Abu, Ayoobul1 aKhan, Mohammad, Islam1 aPillai, Vijayamohanan, K.  uhttps://library.ncl.res.in/content/carbon-nanotube-modified-sodium-dodecyl-sulfate-polyacrylamide-gel-electrophoresis-0