01291nas a2200157   4500008004100000022001400041245014900055210006900204260006700273300001200340490000700352520060800359100002000967700001900987856012701006       2011                        eng d  a1744-309100aPurification, crystallization and X-ray characterization of a Kunitz-type trypsin inhibitor protein from the seeds of chickpea (Cicer arietinum)0 aPurification crystallization and Xray characterization of a Kuni  a111 RIVER ST, HOBOKEN 07030-5774, NJ USAbWILEY-BLACKWELLcJUN  a714-7170 v673 a<p>{A Kunitz-type trypsin inhibitor protein (CPTI) purified from chickpea seeds was estimated to have a molecular mass of 18 kDa on SDS-PAGE. The IC50 value of CPTI was determined to be 2.5 mu g against trypsin. The inhibitory activity of CPTI is 114 TIU (trypsin inhibitory units) per milligram of protein, which is high compared with those of other known Kunitz-type trypsin inhibitors from legumes. CPTI crystallized in three different orthorhombic crystal forms: P2(1)2(1)2 form A, P2(1)2(1)2 form B and P2(1)2(1)2(1). The crystals of P2(1)2(1)2 form angstrom, with unit-cell parameters a = 37.2</p>1 aSharma, Urvashi1 aSuresh, C., G.  uhttps://library.ncl.res.in/content/purification-crystallization-and-x-ray-characterization-kunitz-type-trypsin-inhibitor-0