02028nas a2200193   4500008004100000022001400041245008000055210006900135260007600204300001200280490000700292520133000299100001701629700001601646700002001662700002501682700001901707856010801726       2012                        eng d  a8756-793800aControlled release of nutrients to mammalian cells cultured in shake flasks0 aControlled release of nutrients to mammalian cells cultured in s  aCOMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USAbWILEY-BLACKWELLcJAN  a188-1950 v283 a<p>Though cell culture-based protein production processes are rarely carried out under batch mode of operation, cell line and initial process development operations are usually carried out in batch mode due to simplicity of operation in widely used scale down platforms like shake flasks. Nutrient feeding, if performed, is achieved by bolus addition of concentrated feed solution at different intervals, which leads to large transient increases in nutrient concentrations. One negative consequence is increased waste metabolite production. We have developed a hydrogel-based nutrient delivery system for continuous feeding of nutrients in scale down models like shake flasks without the need for manual feed additions or any additional infrastructure. Continuous delivery also enables maintaining nutrient concentrations at low levels, if desired. The authors demonstrate the use of these systems for continuous feeding of glucose and protein hydrolysate to a suspension Chinese Hamster Ovary (CHO) culture in a shake flask. Glucose feeding achieved using the glucose-loaded hydrogel resulted in a 23% higher integral viable cell density and an 89% lower lactate concentration at the end of the culture when compared with a bolus-feed of glucose. (c) 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2012</p>1 aHegde, Swati1 aPant, Tejal1 aPradhan, Ketaki1 aBadiger, Manohar, V.1 aGadgil, Mugdha  uhttps://library.ncl.res.in/content/controlled-release-nutrients-mammalian-cells-cultured-shake-flasks-0