02364nas a2200193   4500008004100000022001400041245009200055210006900147260007100216300001400287490000700301520165100308100001601959700001801975700001701993700001702010700002202027856012102049       2012                        eng d  a1525-779700aSynthesis of silk fibroin-glycopolypeptide conjugates and their recognition with lectin0 aSynthesis of silk fibroinglycopolypeptide conjugates and their r  a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcNOV  a3695-37020 v133 a<p>Silk fibroin (SF), the natural fibrous protein created by the Bombyx mori silk worm, is being increasingly excellent mechanical strength, high oxygen/water permeability, excellent mechanical strength; high oxygen/water:permeability, and biocompatibility: :It is also well known that surface. modification of SF, With Organic ligands such as the extracellular protein :binding. Arg-Gly-Asp, (RGD) peptides:,: help adhesion and,proliferation. of cells better-a key; requirement for it to function as extracellular. matrices; In this, Work, We have conjugated synthetic glycopolypeptides (GPs) that were synthesized by controlled ring opening polymerization of alpha-manno-lys N-carboxyanhydrides (NCAs) onto SF by using Cu catalyzed click reaction to synthesize 1 a new hybrid :Material (SF GP), Which We believe will have :both the mechanical properties of native SF and the molecular recognition property of the carbohydrates in the GP. By controlling the :amount of GP grafted onto SF, we have made three SF GP conjugates that differ in their ability to assemble into films. SF GP conjugates having a very high content of GP formed completely water soluble brush like polymer that displayed very high affinity toward the lectin concanavalin-A (Con-A) Films cast from SF-GP conjugates using lower amounts of grafted GP were more stable in water, and the stability can be modulated by varying the amount of G grafted. The water insoluble film SF-GP(25) was also found to bind to fluorescently labeled Con A, as was seen by confocal microscopy Such SF-GP hybrid films may be useful as mimics of extracellular matrices for tissue engineering:</p>1 aDas, Soumen1 aPati, Debasis1 aTiwari, Neha1 aNisal, Anuya1 aGupta, Sayam, Sen  uhttps://library.ncl.res.in/content/synthesis-silk-fibroin-glycopolypeptide-conjugates-and-their-recognition-lectin-0