01811nas a2200229   4500008004100000022001400041245009700055210006900152260006700221300001400288490000700302520091800309653003001227653003201257653003001289653004001319653004301359100002001402700001601422700001901438856012401457       2012                        eng d  a8756-793800aIn situ pH maintenance for mammalian cell cultures in shake flasks and tissue culture flasks0 aIn situ pH maintenance for mammalian cell cultures in shake flas  a111 RIVER ST, HOBOKEN 07030-5774, NJ USAbWILEY-BLACKWELLcNOV  a1605-16100 v283 a<p>pH in animal cell cultures decreases due to production of metabolites like lactate. pH control via measurement and base addition is not easily possible in small-scale culture formats like tissue-culture flasks and shake flasks. A hydrogel-based system is reported for in situ pH maintenance without pH measurement in such formats, and is demonstrated to maintain pH between 6.8 and 7.2 for a suspension CHO cell line in CD CHO medium and between 7.3 and 7.5 for adherent A549 cells in DMEM:F12 containing 10% FBS. This system for pH maintenance, along with our previous report of hydrogels for controlled nutrient delivery in shake flasks can allow shake flasks to better mimic bioreactor-based fed batch operation for initial screening during cell line and process development for recombinant protein production in mammalian cells. (C) 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012</p>10ahigh throughput screening10apH control for cell culture10apH control in shake flask10apH control in tissue culture flasks10asmall scale platforms for cell culture1 aPradhan, Ketaki1 aPant, Tejal1 aGadgil, Mugdha  uhttps://library.ncl.res.in/content/situ-ph-maintenance-mammalian-cell-cultures-shake-flasks-and-tissue-culture-flasks-0