02105nas a2200253   4500008004100000022001400041245016500055210006900220260009900289300001400388490000800402520111200410653000901522653001801531653002901549653001301578653002001591100002101611700002401632700002101656700002401677700002201701856012801723       2012                        eng d  a0308-814600aDiscovering bisdemethoxycurcumin from curcuma longa rhizome as a potent small molecule inhibitor of human pancreatic alpha-amylase, a target for type-2 diabetes0 aDiscovering bisdemethoxycurcumin from curcuma longa rhizome as a  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcDEC  a2638-26420 v1353 a<p>Curcuma longa rhizome is used extensively in culinary preparations in Far East and South-East Asia. Health benefits of curcuminoids from C. longa as antioxidants, anti-cancer and anti-inflammatory molecules have been well documented. We report here for the first time that Bisdemethoxycurcumin (BDMC) from C. longa, acts as an inhibitor to inactivate human pancreatic alpha-amylase, a therapeutic target for oral hypoglycemic agents in type-2 diabetes. Bioactivity guided isolation of rhizome isopropanol extract led to the identification by HPLC and NMR of BDMC as a lead small molecule inhibitor of porcine and human pancreatic alpha-amylase with an IC50 value of 0.026 and 0.025 mM, respectively. Kinetic analysis revealed that using starch as the substrate, HPA exhibited an uncompetitive mode of inhibition with an apparent K-i of 3.0 mu M. The study gains importance as BDMC could be a good drug candidate in development of new inhibitors of HPA and of functional foods for controlling starch digestion in order to reduce post-prandial hyperglycemia. (C) 2012 Elsevier Ltd. All rights reserved.</p>10aBDMC10aCurcuma longa10aHuman pancreatic amylase10akinetics10aType-2 diabetes1 aPonnusamy, Sudha1 aZinjarde, Smita, S.1 aBhargava, Shobha1 aRajamohanan, P., R.1 aRaviKumar, Ameeta  uhttps://library.ncl.res.in/content/discovering-bisdemethoxycurcumin-curcuma-longa-rhizome-potent-small-molecule-inhibitor-0