02407nas a2200253   4500008004100000022001400041245016000055210006900215260007700284300001200361490000700373520141300380653002301793653001701816653001701833653003901850100002301889700002501912700002301937700002401960700002001984700001802004856013102022       2013                        eng d  a0038-905600aOptimization of the production of a maltooligosaccharides producing amylase from the alkalophilic streptomyces lonarensis strain NCL 716 using SVR modeling0 aOptimization of the production of a maltooligosaccharides produc  aBOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANYbWILEY-V C H VERLAG GMBHcJAN  a179-1850 v653 a<p>The Streptomyces lonarensis strain NCL 716 hydrolyses starch to produce a mixture of maltotriose (G3) and maltotetraose (G4) along with maltose (G2). The objective of the present work was to determine an optimum cost effective media composition for the production of a-amylase from this strain. The most influential factor was found to be starch while the least influential factor found was peptone by PlackettBurman method. Peptone amount was kept constant throughout the fermentation. Peptone, which is one of the expensive media components was used at a concentration of 1?g/L, which made the optimum media composition cost effective. A support vector regression-based process model was developed for approximating the non-linear relationship between the fermentation operating variables and the a-amylase yield. Multicanonical Jump Walk Annealing, a stochastic optimization technique is used to obtain optimal operating variables to maximize amylase yield. The maximum amylase activity thus obtained was in good agreement with the experimental values at the optimized levels. The optimum media composition obtained by this method was: yeast extract: 4.53?g/L, starch: 20.246?g/L, K2HPO4: 0.0827%, MgSO4: 0.15%, peptone: 1?g/L. A maximum enzyme activity of 297?U/mL, which was achieved using the above approaches compares well with the activity of reported amylases producing maltooligosaccharides.</p>10aAlkaline a-amylase10aOptimization10aStreptomyces10aSupport vector regression analysis1 aSharma, Trupti, K.1 aBhadane, Vaibhav, A.1 aKumar, Lalitha, S.1 aRele, Meenakshi, V.1 aBhawar, Gajanan1 aRahman, Imran  uhttps://library.ncl.res.in/content/optimization-production-maltooligosaccharides-producing-amylase-alkalophilic-streptomyces-0