01456nas a2200193   4500008004100000022001400041245018200055210006900237260011000306300001200416490000700428520057600435100002401011700002201035700002401057700002401081700002601105856013101131       2013                        eng d  a1477-052000aSynthesis and structural studies of S-type/N-type-locked/frozen nucleoside analogues and their incorporation in RNA-selective, nuclease resistant 2 `-5 ` linked oligonucleotides0 aSynthesis and structural studies of StypeNtypelockedfrozen nucle  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcNOV  a746-7570 v113 a<p>2'-endo locked or frozen (S-type)/3'-endo locked or frozen (N-type) nucleoside analogues were synthesized. Conformational analysis based on (3)J(HH) and NOE measurements is presented which is further confirmed by X-ray crystal structural studies. 2'-5' isoDNA oligonucleotides (ON) were synthesized using these modified nucleoside analogues and UV-T-m studies of the resultant 2'-5' isoDNA : RNA duplexes reflect the site-and sequence-dependent effects and confirm that the S-type sugar conformations were preferred over the N-type sugar geometry in such duplexes.</p>1 aErande, Namrata, D.1 aGunjal, Anita, D.1 aFernandes, Moneesha1 aGonnade, Rajesh, G.1 aKumar, Vaijayanti, A.  uhttps://library.ncl.res.in/content/synthesis-and-structural-studies-s-typen-type-lockedfrozen-nucleoside-analogues-and-their-0