02522nas a2200265   4500008004100000022001400041245007700055210006900132260007300201300001200274490000800286520164000294653001601934653000901950653001001959653001401969653002701983653002902010100002802039700001802067700001802085700001802103700002702121856010802148       2013                        eng d  a0927-776500aBiosynthesis of Se nanoparticles and its effect on UV-induced DNA damage0 aBiosynthesis of Se nanoparticles and its effect on UVinduced DNA  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAR  a261-2660 v1033 a<p>This paper reports, an environmentally benign procedure of synthesis and characterizations of selenium nanoparticles and their protective effect against UV-induced DNA damage activities. An aqueous leaf extract of lemon plant was used as a precursor for synthesis of colloidal selenium nanoparticles. Resulting nanoparticles were characterized using UV-vis spectrophotometer, photoluminescence, TEM, EDAX, FT-IR and XRD, respectively. Selenium colloidal solution exhibited an absorption maximum at 395 nm and produced an emission maximum at 525 nm. Transmission electron microscopy followed by selected area electron diffraction pattern analysis indicated the formation of spherical, polydispersed, crystalline, selenium nanoparticles of diameter ranging from (similar to 60 to 80 nm). X-ray diffraction studies showed the formation of 111, 2 0 0 and 220 planes of face-centered cubic (fcc) selenium. EDAX analysis confirmed the presence of selenium in nanosphere. Fourier transformed infrared spectroscopic investigation reveled the involvement of carboxyl (-C=0), hydroxyl (-OH), amine (-NH) functional group of lemon plant extract in preparation of selenium nano particles. MU assay as well single cell gel electrophoresis assay or comet assay revealed that synthesized selenium nanoparticles, caused less cell death of lymphocytes and prevented DNA damage, when cells were exposed to UVB. The fluorescent property of selenium nanopartides can be used as diagnostic agent. Further, their anti DNA damaging property can be investigated as a chemotherapeutic agent in cancer therapy. (C) 2012 Elsevier B.V. All rights reserved.</p>10aComet assay10aEDAX10aFT-IR10aMTT assay10aSelenium nanoparticles10aUV-vis spectrophotometer1 aPrasad, Kumar, Suranjit1 aPatel, Hirnee1 aPatel, Tirtha1 aPatel, Khusbu1 aSelvaraj, Kaliaperumal  uhttps://library.ncl.res.in/content/biosynthesis-se-nanoparticles-and-its-effect-uv-induced-dna-damage-0