01696nas a2200205   4500008004100000022001400041245012800055210006900183260010600252300001400358490000700372520082700379653002701206653003701233653001601270653003101286100002001317700002601337856012701363       2013                        eng d  a0968-089600aC-3 `-endo-puckered pyrrolidine containing PNA has favorable geometry for RNA binding: novel ethano locked PNA (ethano-PNA)0 aC3 endopuckered pyrrolidine containing PNA has favorable geometr  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcJUL  a4092-41010 v213 a<p>A novel peptide nucleic acid (PNA) analogue is designed with a constraint in the aminoethyl segment of the aegPNA backbone so that the dihedral angle beta is restricted within 60-80 degrees, compatible to form PNA:RNA duplexes. The designed monomer is further functionalized with positively charged amino-/guanidino-groups. The appropriately protected monomers were synthesized and incorporated into aegPNA oligomers at predetermined positions and their binding abilities with cDNA and RNA were investigated. A single incorporation of the modified PNA monomer into a 12-mer PNA sequence resulted in stronger binding with complementary RNA over cDNA. No significant changes in the CD signatures of the derived duplexes of modified PNA with complementary RNA were observed. (C) 2013 Elsevier Ltd. All rights reserved.</p>10aAntisense therapeutics10aConformationally constrained PNA10aDiagnostics10aPeptide nucleic acid (PNA)1 aBanerjee, Anjan1 aKumar, Vaijayanti, A.  uhttps://library.ncl.res.in/content/c-3-endo-puckered-pyrrolidine-containing-pna-has-favorable-geometry-rna-binding-novel-0