01871nas a2200181   4500008004100000022001400041245015600055210006900211260009100280300001600371490000600387520106700393100002001460700001801480700004301498700001701541856013101558       2013                        eng d  a1932-620300aIsolation, purification and characterization of vinblastine and vincristine from endophytic fungus fusarium oxysporum isolated from catharanthus roseus0 aIsolation purification and characterization of vinblastine and v  a1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USAbPUBLIC LIBRARY SCIENCEcSEP  aUNSP e718050 v83 a<p>Endophytic fungi reside in a symbiotic fashion inside their host plants, mimic their chemistry and interestingly, produce the same natural products as their hosts and are thus being screened for the production of valuable compounds like taxol, camptothecin, podophyllotoxin, etc. Vinblastine and vincristine are excellent anti-cancer drugs but their current production using plants is non-abundant and expensive. In order to make these drugs readily available to the patients at affordable prices, we isolated the endophytic fungi from Catharanthus roseus plant and found a fungus AA-CRL-6 which produces vinblastine and vincristine in appreciable amounts. These drugs were purified by TLC and HPLC and characterized using UV-Vis spectroscopy, ESI-MS, MS/MS and H-1 NMR. One liter of culture filtrate yielded 76 mu g and 67 mu g of vinblastine and vincristine respectively. This endophytic fungal strain was identified as Fusarium oxysporum based upon its cultural and morphological characteristics and internal transcribed spacer (ITS) sequence analysis.</p>1 aKumar, Ashutosh1 aPatil, Deepak1 aRajamohanan, Pattuparambil, Ramanpilla1 aAhmad, Absar  uhttps://library.ncl.res.in/content/isolation-purification-and-characterization-vinblastine-and-vincristine-endophytic-fungus-0