02032nas a2200181   4500008004100000022001400041245013500055210006900190260009100259300001100350490000600361520125800367100002801625700002301653700002001676700002501696856012901721       2013                        eng d  a1932-620300aAlterations in the helicoverpa armigera midgut digestive physiology after ingestion of pigeon pea inducible leucine aminopeptidase0 aAlterations in the helicoverpa armigera midgut digestive physiol  a1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USAbPUBLIC LIBRARY SCIENCEcSEP  ae748890 v83 a<p>Jasmonate inducible plant leucine aminopeptidase (LAP) is proposed to serve as direct defense in the insect midgut. However, exact functions of inducible plant LAPs in the insect midgut remain to be estimated. In the present investigation, we report the direct defensive role of pigeon pea inducible LAP in the midgut of Helicoverpa armigera (Lepidoptera: Noctuidae) and responses of midgut soluble aminopeptidases and serine proteinases upon LAP ingestion. Larval growth and survival was significantly reduced on the diets supplemented with pigeon pea LAP. Aminopeptidase activities in larvae remain unaltered in presence or absence of inducible LAP in the diet. On the contrary, serine proteinase activities were significantly decreased in the larvae reared on pigeon pea LAP containing diet as compared to larvae fed on diet without LAP. Our data suggest that pigeon pea inducible LAP is responsible for the degradation of midgut serine proteinases upon ingestion. Reduction in the aminopeptidase activity with LpNA in the H. armigera larvae was compensated with an induction of aminopeptidase activity with ApNA. Our findings could be helpful to further dissect the roles of plant inducible LAPs in the direct plant defense against herbivory.</p>1 aLomate, Purushottam, R.1 aJadhav, Bhakti, R.1 aGiri, Ashok, P.1 aHivrale, Vandana, K.  uhttps://library.ncl.res.in/content/alterations-helicoverpa-armigera-midgut-digestive-physiology-after-ingestion-pigeon-pea-0