02840nas a2200241   4500008004100000022001400041245009000055210006900145260008100214300000700295490000700302520195400309653001902263653002402282653002102306653002602327653002902353100002602382700002802408700002002436700002502456856011702481       2013                        eng d  a1471-209100aCharacterization of a chemostable serine alkaline protease from Periplaneta americana0 aCharacterization of a chemostable serine alkaline protease from   a236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLANDbBIOMED CENTRAL LTDcNOV  a320 v143 a<p>Background: Proteases are important enzymes involved in numerous essential physiological processes and hold a strong potential for industrial applications. The proteolytic activity of insects' gut is endowed by many isoforms with diverse properties and specificities. Thus, insect proteases can act as a tool in industrial processes. Results: In the present study, purification and properties of a serine alkaline protease from Periplaneta americana and its potential application as an additive in various bio-formulations are reported. The enzyme was purified near to homogeneity by using acetone precipitation and Sephadex G-100 gel filtration chromatography. Enzyme activity was increased up to 4.2 fold after gel filtration chromatography. The purified enzyme appeared as single protein-band with a molecular mass of similar to 27.8 kDa in SDS-PAGE. The optimum pH and temperature for the proteolytic activity for purified protein were found around pH 8.0 and 60 degrees C respectively. Complete inhibition of the purified enzyme by phenylmethylsulfonyl fluoride confirmed that the protease was of serine-type. The purified enzyme revealed high stability and compatibility towards detergents, oxidizing, reducing, and bleaching agents. In addition, enzyme also showed stability towards organic solvents and commercial detergents. Conclusion: Several important properties of a serine protease from P. Americana were revealed. Moreover, insects can serve as excellent and alternative source of industrially important proteases with unique properties, which can be utilized as additives in detergents, stain removers and other bio-formulations. Properties of the P. americana protease accounted in the present investigation can be exploited further in various industrial processes. As an industrial prospective, identification of enzymes with varying essential properties from different insect species might be good approach and bioresource.</p>10aChemostability10aIndustrial catalyst10aInsect proteases10aPeriplaneta americana10aSerine alkaline protease1 aSanatan, Prashant, T.1 aLomate, Purushottam, R.1 aGiri, Ashok, P.1 aHivrale, Vandana, K.  uhttps://library.ncl.res.in/content/characterization-chemostable-serine-alkaline-protease-periplaneta-americana-0