02196nas a2200217   4500008004100000022001400041245009200055210006900147260007100216300001400287490000700301520139700308100001801705700002701723700001901750700001601769700003101785700002301816700001801839856012101857       2014                        eng d  a0006-296000aMorphological effects of G-quadruplex stabilization using a small molecule in zebrafish0 aMorphological effects of Gquadruplex stabilization using a small  a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcFEB  a1117-11240 v533 a<p>Zebrafish (Danio rerio) embryos are transparent and advantageous for studying early developmental changes due to ex utero development, making them an appropriate model for studying gene expression changes as a result of molecular targeting. Zebrafish embryos were injected with a previously reported G-quadruplex selective ligand, and the phenotypic changes were recorded. We report marked discrepancies in the development of intersegmental vessels. In silico analysis determined that the putative G-quadruplex motif occur in the upstream promoter region of the Cdh5 (N-cadherin) gene. A real-time polymerase chain reaction-based investigation indicated that in zebrafish, CDH-2 (ZN-cad) was significantly downregulated in the ligand-treated embryos. Biophysical characterization of the interaction of the ligand with the G-quadruplex motif found in this promoter yielded strong binding and stabilization of the G-quadruplex with this ligand. Hence, we report for the first time the phenotypic impact of G-quadruplex targeting with a ligand in a vertebrate organism. This study has unveiled not only G-quadruplex targeting in non-human animal species but also the potential that G-quadruplexes can provide a ready tool for understanding the phenotypic effects of targeting certain important genes involved in differentiation and developmental processes in a living eukaryotic organism.</p>1 aAgarwal, Tani1 aLalwani, Mukesh, Kumar1 aKumar, Santosh1 aRoy, Saumya1 aChakraborty, Tushar, Kanti1 aSivasubbu, Sridhar1 aMaiti, Souvik  uhttps://library.ncl.res.in/content/morphological-effects-g-quadruplex-stabilization-using-small-molecule-zebrafish-0