01705nas a2200241   4500008004100000022001400041245008800055210006900143260010600212300001400318490000700332520081800339653001101157653001901168653002301187653001101210653002201221100001901243700002101262700002301283700003701306856012001343       2014                        eng d  a0040-403900aLipase mediated separation of triterpene structural isomers, alpha- and beta-amyrin0 aLipase mediated separation of triterpene structural isomers alph  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcMAY  a3122-31250 v553 a<p>Pentacyclic triterpenoids alpha- and beta-amyrin possess a wide range of biological and pharmacological activities. High structural similarity between these two structural isomers makes their chromatographic separation an ineffective and tedious choice. In this study, Candida rugosa lipase catalyzed separation protocol for the isolation of individual isomers has been developed. In the presence of vinyl acetate as the acyl donor, Candida rugosa lipase carried out acetylation of beta-amyrin more efficiently as compared to alpha-amyrin leading to a kinetic separation. The conditions of transesterification reaction were optimized systematically, which was utilized to separate alpha- and beta-amyrin from a mixture obtained from the latex of Plumeria obtusa. (C) 2014 Elsevier Ltd. All rights reserved.</p>10aAmyrin10aCandida rugosa10aKinetic separation10aLipase10aStructural isomer1 aHaldar, Saikat1 aKale, Balaji, S.1 aJadhav, Dipesh, D.1 aThulasiram, Hirekodathakallu, V.  uhttps://library.ncl.res.in/content/lipase-mediated-separation-triterpene-structural-isomers-alpha-and-beta-amyrin-0