01977nas a2200265   4500008004100000022001400041245021300055210006900268260007900337300001000416490001000426520089900436653002301335653002501358653002001383653001901403653001801422100003101440700002401471700002401495700001901519700001901538700002501557856012901582       2014                        eng d  a0141-022900aCo-immobilized glucose oxidase and beta-galactosidase on bovine serum albumin coated allyl glycidyl ether (AGE)-ethylene glycol dimethacrylate (EGDM) copolymer as a biosensor for lactose determination in milk0 aCoimmobilized glucose oxidase and betagalactosidase on bovine se  a360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USAbELSEVIER SCIENCE INCcOCT  a67-730 v64-653 a<p>Bovine serum albumin (BSA) was adsorbed on allyl glycidyl ether (AGE)-ethylene glycol dimethacrylate (EGDM) copolymer with 25% crosslink density (AGE-25) at pH 8.0 (16% w/w). The amino, thiol and carboxylic acid functional groups available on protein coated surface were utilized for covalent immobilization of glucose oxidase and beta-galactosidase, both independently, and in a step-wise manner on the same matrix, with no more than 10% loss of enzyme activity during immobilization. Glutaraldehyde cross-linking after immobilization provided stable enzyme preparations. The pH-optima of the immobilized enzymes were similar to those for free enzyme but their thermal stability was vastly improved. The co-immobilized enzyme support was used as a biosensor for determination of lactose in milk with excellent reproducibility and reusability. (C) 2014 Elsevier Inc. All rights reserved.</p>10abeta-Galactosidase10aBovine serum albumin10aGlucose oxidase10aImmobilization10aLactose assay1 aJasti, Lakshmi, Swarnalath1 aDola, Sandhya, Rani1 aFadnavis, Nitin, W.1 aAddepally, Uma1 aDaniels, Siona1 aPonrathnam, Surendra  uhttps://library.ncl.res.in/content/co-immobilized-glucose-oxidase-and-beta-galactosidase-bovine-serum-albumin-coated-allyl-0