01981nas a2200181   4500008004100000022001400041245010600055210006900161260011000230300001400340490000600354520122700360100002001587700002001607700001701627700002601644856012901670       2015                        eng d  a2046-206900aBiocatalyst development for lactic acid production at acidic pH using inter-generic protoplast fusion0 aBiocatalyst development for lactic acid production at acidic pH   aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcDEC  a2024-20310 v53 a<p>Global warming and environmental problems force us to develop sustainable processes based on the use of biocatalysts that are eco-friendly with the least potential toxicity. Lactic acid fermentation at neutral pH generates a large amount of gypsum during down-stream processing. Hence it is essential to develop Lactobacillus strains which produce lactic acid at acidic pH thus making the whole downstream process environmentally friendly. Fusant F3 was generated using protoplast fusion between Lactobacillus delbrueckii Mut Uc-3 and Acetobacter pasteurianus NCIM 2314 on solid media at pH 4.0. Fusant F3 was further treated by UV irradiation to generate a mutant, FM1, with improvements in acid tolerance which produced five-fold more lactic acid than the parent strain at acidic pH. The molecular studies using RAPD markers demonstrated that the fusant is derived from both the parental strains, Acetobacter and Lactobacillus and the mutant is derived from the fusant. The utilization of such acid tolerant strains could be able to produce free lactic acid at acidic pH without using neutralizing agents and will offer an effective means for designing environmentally benign processes for lactic acid production.</p>1 aSinghvi, Mamata1 aGurjar, Gayatri1 aGupta, Vidya1 aGokhale, Digambar, V.  uhttps://library.ncl.res.in/content/biocatalyst-development-lactic-acid-production-acidic-ph-using-inter-generic-protoplast-0