02316nas a2200193   4500008004100000022001400041245015600055210006900211260005700280300001200337490000700349520151600356100002301872700002701895700002301922700002201945700002701967856012801994       2015                        eng d  a0971-589400aAbiotic stress induces change in cinnamoyl CoA reductase (CCR) protein abundance and lignin deposition in developing seedlings of Leucaena leucocephala0 aAbiotic stress induces change in cinnamoyl CoA reductase CCR pro  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcAPR  a197-2050 v213 a<p>Aboitic stress such as drought and salinity are class of major threats, which plants undergo through their lifetime. Lignin deposition is one of the responses to such abiotic stresses. The gene encoding Cinnamoyl CoA Reductase (CCR) is a key gene for lignin biosynthesis, which has been shown to be over-expressed under stress conditions. In the present study, developing seedlings of Leucaena leucocephala (Vernacular name: Subabul, White popinac) were treated with 1 % mannitol and 200 mM NaCl to mimic drought and salinity stress conditions, respectively. Enzyme linked immunosorbant assay (ELISA) based expression pattern of CCR protein was monitored coupled with Phlorogucinol/HCl activity staining of lignin in transverse sections of developing L. leucocephala seedlings under stress. Our result suggests a differential lignification pattern in developing root and stem under stress conditions. Increase in lignification was observed in mannitol treated stems and corresponding CCR protein accumulation was also higher than control and salt stress treated samples. On the contrary CCR protein was lower in NaCl treated stems and corresponding lignin deposition was also low. Developing root tissue showed a high level of CCR content and lignin deposition than stem samples under all conditions tested. Overall result suggested that lignin accumulation was not affected much in case of developing root however developing stems were significantly affected under drought and salinity stress condition.</p>1 aSrivastava, Sameer1 aVishwakarma, Rishi, K.1 aArafat, Yasir, Ali1 aGupta, Sushim, K.1 aKhan, Bashir, Mohammad  uhttps://library.ncl.res.in/content/abiotic-stress-induces-change-cinnamoyl-coa-reductase-ccr-protein-abundance-and-lignin-0