01999nas a2200181   4500008004100000022001400041245008200055210006900137260007100206300001400277490000800291520132600299100002101625700001901646700002501665700001801690856010901708       2015                        eng d  a1520-610600aHuman telomeric RNA G-quadruplex response to point mutation in the G-quartets0 aHuman telomeric RNA Gquadruplex response to point mutation in th  a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcAPR  a4617-46270 v1193 a<p>Many putative G-quadruplex forming sequences have been predicted to exist in the human genome and transcriptome. As these sequences are subject to point mutations or SNPs (single nucleotide polymorphisms) during the course of evolution, we attempt to understand impact of these mutations in context of RNA G-quadruplex formation using human telomeric RNA (TERRA) as a model sequence. Our studies suggest that G-quadruplex stability is sensitive to substitution of the guanines comprising G-quartets. While central G-quartet plays a crucial role in maintaining the DNA G-quadruplex stability as evident in literature, there is equal importance of three G-quartets in the stability of RNA quadruplex structure. The work here highlights the alterations in the G-quartet are detrimental to the integrity of overall RNA G-quadruplex structure. Furthermore, TmPyP4 molecules are shown to exhibit similar binding behavior toward telomeric RNA G-quadruplex harboring base substitutions employing CD titrations and isothermal titration calorimetry; well indicating that mutation does not influence TmPyP4 recognition ability as it affects the stability of RNA G-quadruplex. Thus, our study implicates that mutation in G-quartets causes destabilization of RNA G-quadruplex without affecting its trans factor binding ability.</p>1 aAgarwala, Prachi1 aKumar, Santosh1 aPandey, Satyaprakash1 aMaiti, Souvik  uhttps://library.ncl.res.in/content/human-telomeric-rna-g-quadruplex-response-point-mutation-g-quartets-0