02095nas a2200241   4500008004100000022001400041245016200055210006900217260009500286300001200381490000800393520114900401653002001550653002101570653002101591653001501612653001301627653001901640653002401659100002601683700002101709856012301730       2015                        eng d  a0888-754300aComparison of codon usage bias across leishmania and trypanosomatids to understand mRNA secondary structure, relative protein abundance and pathway functions0 aComparison of codon usage bias across leishmania and trypanosoma  a525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USAbACADEMIC PRESS INC ELSEVIER SCIENCEcOCT  a232-2410 v1063 a<p>Understanding the variations in gene organization and its effect on the phenotype across different Leishmania species, and to study differential clinical manifestations of parasite within the host, we performed large scale analysis of codon usage patterns between Leishmania and other known Trypanosomatid species. We present; the causes and consequences of codon usage bias in Leishmania genomes with respect to mutational pressure, translational selection and amino acid composition bias. We establish GC bias at wobble position that governs codon usage bias across Leishmania species, rather than amino acid composition bias. We found that, within Leishmania, homogenous codon context coding for less frequent amino acid pairs and codons avoiding formation of folding structures in mRNA are essentially chosen. We predicted putative differences in global expression between genes belonging to specific pathways across Leishmania. This explains the role of evolution in shaping the otherwise conserved genome to demonstrate species-specific function-level differences for efficient survival. (C) 2015 Elsevier Inc. All rights reserved.</p>10aAmino acid bias10aBase composition10aCodon usage bias10aLeishmania10aMutation10aSelection bias10aSpecies specificity1 aSubramanian, Abhishek1 aSarkar, Ram, Rup  uhttps://library.ncl.res.in/content/comparison-codon-usage-bias-across-leishmania-and-trypanosomatids-understand-mrna-0