02365nas a2200241   4500008004100000022001400041245012400055210006900179260010200248300001200350490000700362520138400369653001801753653003401771653004401805653003001849653003801879100002001917700002201937700001901959700002101978856012401999       2015                        eng d  a0250-599100aTemporal protein expression pattern in intracellular signalling cascade during T-cell activation: a computational study0 aTemporal protein expression pattern in intracellular signalling   aC V RAMAN AVENUE, SADASHIVANAGAR, P B \#8005, BANGALORE 560 080, INDIAbINDIAN ACAD SCIENCEScOCT  a769-7890 v403 a<p>Various T-cell co-receptor molecules and calcium channel CRAC play a pivotal role in the maintenance of cell's functional responses by regulating the production of effector molecules (mostly cytokines) that aids in immune clearance and also maintaining the cell in a functionally active state. Any defect in these co-receptor signalling pathways may lead to an altered expression pattern of the effector molecules. To study the propagation of such defects with time and their effect on the intracellular protein expression patterns, a comprehensive and largest pathway map of T-cell activation network is reconstructed manually. The entire pathway reactions are then translated using logical equations and simulated using the published time series microarray expression data as inputs. After validating the model, the effect of in silico knock down of co-receptor molecules on the expression patterns of their downstream proteins is studied and simultaneously the changes in the phenotypic behaviours of the T-cell population are predicted, which shows significant variations among the proteins expression and the signalling routes through which the response is propagated in the cytoplasm. This integrative computational approach serves as a valuable technique to study the changes in protein expression patterns and helps to predict variations in the cellular behaviour.</p>10aBoolean model10aco-receptors and CRAC channel10asynchronous and asynchronous simulation10aT-cell signalling pathway10atemporal gene expression patterns1 aGanguli, Piyali1 aChowdhury, Saikat1 aBhowmick, Rupa1 aSarkar, Ram, Rup  uhttps://library.ncl.res.in/content/temporal-protein-expression-pattern-intracellular-signalling-cascade-during-t-cell-0