@article { ISI:000254697200009,
	title = {Purification and characterization of a T-antigen specific lectin from the coelomic fluid of a marine invertebrate, sea cucumber (Holothuria scabra)},
	journal = {Fish \& Shellfish Immunology},
	volume = {24},
	number = {4},
	year = {2008},
	month = {APR},
	pages = {450-458},
	publisher = {ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD},
	address = {24-28 OVAL RD, LONDON NW1 7DX, ENGLAND},
	abstract = {<p>A novel lectin was purified from the coelomic fluid of the sea cucumber Holothuria scabra (HSL), subjected to bacterial challenge. HSL is a monomeric glycoprotein of molecular mass 182 kDa. The lectin is highly thermostable as it retains full activity for 1 h at 80 degrees C. Further, the hemagglutination activity of HSL is unaffected by pH in the range 2-11. Unlike other lectins purified from marine invertebrates, the hemagglutination activity of HSL does not require any divalent metal ions. The affinity profile of HSL was studied by a combination of hemagglutination inhibition and fluorescence spectroscopy. HSL binds to desialylated glycoproteins, Me alpha Gal, T-antigen and T (alpha-ser)-antigen with a distinction between beta 1-4 and beta 1-3 linkages. Me alpha-T-antigen was a potent ligand having highest affinity (K-a 8.32 x 10(7) M-1). Monosaccharide binding is enthalphically driven while disaccharide binding involves both entropic and enthalpic contributions. (c) 2008 Elsevier Ltd. All rights reserved.</p>},
	keywords = {Fluorescence spectroscopy, Holothuria scabra, Lectin, marine invertebrate, Thermodynamic properties},
	issn = {1050-4648},
	doi = {10.1016/j.fsi.2008.01.002},
	author = {Gowda, Nagaraj M. and Goswami, Usha and Khan, Mohammad Islam}
}
@article {46699,
	title = {Characterization of multidrug resistance in Vibriospecies isolated from marine invertebrates from Andaman Sea},
	journal = {3 Biotech},
	volume = {10},
	year = {2020},
	month = {SEP},
	pages = {456},
	type = {Article},
	abstract = {<p>This study describes the abundance of multidrug-resistantVibriosassociated with marine invertebrate hosts from the Andaman Sea, India. Thirty-eightVibriostrains were isolated from surface mucus layers of coralPorites,Goniastrea, Pocillopora, Fungia, and eggs of spiny lobster (Panulirus penicillatus). Phenotypically, the majority of strains exhibited growth at a wide range of temperatures, salt tolerance, and diverse nutritional requirements. All the strains had more than 97\% 16S rRNA sequence similarity with type species of the genusVibriowhereVibrio fortis, andVibrio alginolyticuswere predominant. Multilocus Sequence Analysis (MLSA) using eight housekeeping genes namelyftsZ,gapA,gyrB,mreB,pyrH,recA,rpoA,andtopAdistributed the strains into 6 reported clades i.e.,Harveyi, Ponticus, Nereis, Orientalis, Splendidus,andMediterraneiwhere nearly half of the total strains represented the cladeHarveyi, followed by the cladeSplendidus.Likewise, the PFGE profile indicated genomic heterogeneity among the strains resulting in their distribution in five major clusters. Resistance to different antimicrobials was tested following the disc diffusion method where all strains were found susceptible to chloramphenicol (30 mu g) and resistant to streptomycin (10 mu g), vancomycin (30 mu g), sulfamethoxazole-trimethoprim (25 mu g). Moreover, the resistant phenotype to other antimicrobials confirmed the abundance of multidrug resistance strains in this marine environment.</p>
},
	keywords = {Drug-resistant, Genetic diversity, marine invertebrate, Multilocus sequence analysis, Vibrio},
	issn = {2190-572X},
	doi = {10.1007/s13205-020-02445-5},
	author = {Kumari, Prabla and Poddar, Abhijit and Das, Subrata K.}
}
