@article { ISI:000272903900005,
	title = {Differential elicitation of an aspartic protease inhibitor: regulation of endogenous protease and initial events in germination in seeds of vigna radiata},
	journal = {Peptides},
	volume = {30},
	number = {12},
	year = {2009},
	month = {DEC},
	pages = {2118-2126},
	publisher = {ELSEVIER SCIENCE INC},
	address = {360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA},
	abstract = {<p>Plant aspartic proteases are of recent origin with their physiological significance in crucial processes emerging. Reports on the significance of aspartic protease inhibitors and their endogenous proteases in seeds of plants are scanty. This paper reports the purification of an aspartic protease inhibitor from the seeds of Vigna radiata, its control of the endogenous aspartic protease and their subsequent role in the early germination events. The role of the aspartic protease inhibitor and the enzyme in initial stages of germination of V. radiata has been tracked by differential timed expression and germination assays. The expression pattern revealed maximum expression of the inhibitor in the dormant seeds while the enzyme was predominant in the germinating seeds. Their expression patterns and interactions indicate their significance in initiation of germination. The expression of other classes of proteases was monitored during germination and a model predicting the events occurring during proteolysis of the storage protein in germination is hypothesized. The inhibitor was a linear, hydrophobic, pH stable and thermostable peptide with molecular weight of 1660 Da. The purified inhibitor showed a pI of 4.36 with the sequence as AEIYN KDGNK LDLYG. The inhibitor was found to be stable in a broad range of pH from 2 to 10 with an optimum of 3.0. The half-life of VrAPI at 100 degrees C was 30 min whereas the maximum activity was observed at 37 degrees C. The initial kinetic analysis of the inhibitor against the enclogenous protease showed an IC50 value of 11 nM while the value of the inhibition rate constant K-i was 34 x 10(-9) M. (C) 2009 Elsevier Inc. All rights reserved.</p>},
	keywords = {Aspartic protease, Plant proteases, Protease inhibitors, Vigna radiata},
	issn = {0196-9781},
	doi = {10.1016/j.peptides.2009.08.024},
	author = {Kulkarni, Aarohi and Rao, Mala}
}
@article { ISI:000377826000008,
	title = {Biogenic green synthesis of monodispersed gum kondagogu (Cochlospermum gossypium) iron nanocomposite material and its application in germination and growth of mung bean (Vigna radiata) as a plant model},
	journal = {IET Nanobiotechnology},
	volume = {10},
	number = {3},
	year = {2016},
	month = {JUN},
	pages = {141-146},
	publisher = {INST ENGINEERING TECHNOLOGY-IET},
	address = {MICHAEL FARADAY HOUSE SIX HILLS WAY STEVENAGE, HERTFORD SG1 2AY, ENGLAND},
	abstract = {<p>An eco-friendly green and one-pot synthesis of highly monodispersed iron (Fe) nanoparticles (NPs) by using a natural biopolymer, gum kondagogu (GK) as reducing and capping agent is proposed. The NPs synthesised were characterised by ultra-violet-visible spectroscopy, transmission electron microscopy, scanning electron microscopy and X-ray diffraction. As the concentration of gum and time increases, the intensity of NPs formation increased. The NPs were highly monodispersed with uniform circular shapes of 2-6 nm in size. The formed NPs were crystalline in nature which was confirmed by diffraction analysis. The conversion ratio of Fe ionic form to NPs was 21\% which was quantified by inductively coupled plasma mass spectroscopy (ICP-MS). Fe is essential for plant growth and development. A study was conducted to examine the effect of these NPs on the growth of mung bean (Vigna radiata). The radical length and biomass was increased in seeds exposed to Fe NPs than the ions. The uptake of Fe NPs by the sprouts was also quantified by ICP-MS, in which Fe was more in mung bean seeds exposed to NPs. The -amylase activity was increased in the seeds exposed to NPs. The observed increase in the biomass by Fe NPs and seed germination may facilitate its application in the agriculture as an important cost-effective method for plant growth.</p>},
	keywords = {alpha-amylase activity, Biogenic green synthesis, biological techniques, Biomass, Cochlospermum gossypium, diffraction analysis, enzymes, Fe, gum concentration, high-monodispersed iron nanoparticles, inductively coupled plasma mass spectroscopy, ionic-to-nanoparticle iron conversion ratio, iron, monodispersed gum kondagogu, monodispersed gum kondagogu iron nanocomposite material, mung bean germination, mung bean growth, Nanobiotechnology, Nanocomposites, nanoparticle formation, Nanoparticles, natural biopolymer, plant growth, plant model, scanning electron microscopy, size 2 nm to 6 nm, transmission electron microscopy, ultraviolet spectra, ultraviolet-visible spectroscopy, Vigna radiata, visible spectra, X-ray diffraction},
	issn = {1751-8741},
	doi = {10.1049/iet-nbt.2015.0112},
	author = {Raju, Dugyala and Mehta, Urmil J. and Beedu, Sashidhar Rao}
}
