@article { ISI:000361828000068,
	title = {Conversion of dried aspergillus candidus mycelia grown on waste whey to biodiesel by in situ acid transesterification},
	journal = {Bioresource Technology},
	volume = {197},
	year = {2015},
	month = {DEC},
	pages = {502-507},
	publisher = {ELSEVIER SCI LTD},
	address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND},
	abstract = {<p>This study reports optimization of the transesterification reaction step on dried biomass of an oleaginous fungus Aspergillus candidus grown on agro-dairy waste, whey. Acid catalyzed transesterification was performed and variables affecting esterification, viz., catalyst methanol and chloroform concentrations, temperature, time, and biomass were investigated. Statistical optimization of the transesterification reaction using Plackett-Burman Design showed biomass to be the predominant factor with a 12.5-fold increase in total FAME from 25.6 to 320 mg. Studies indicate that the transesterification efficiency in terms of conversion is favored by employing lower biomass loadings. A. candidus exhibited FAME profiles containing desirable saturated (30.2\%), monounsaturated (31.5\%) and polyunsaturated methyl esters (38.3\%). The predicted and experimentally determined biodiesel properties (density, kinematic viscosity, iodine value, cetane number, TAN, water content, total and free glycerol) were in accordance with international (ASTM D6751, EN 14214) and national (IS 15607) standards. (C) 2015 Published by Elsevier Ltd.</p>},
	keywords = {Aspergillus candidus, Biodiesel, In situ acid transesterification, Plackett-Burman Design, Whey},
	issn = {0960-8524},
	doi = {10.1016/j.biortech.2015.07.118},
	author = {Kakkad, Hardik and Khot, Mahesh and Smita S. Zinjarde and RaviKumar, Ameeta and Kumar, V. Ravi and Kulkarni, B. D.}
}
@article {46098,
	title = {Use of Plackett-Burman design for enhanced phytase production by Williopsis saturnus NCIM 3298 for applications in animal feed and ethanol production},
	journal = {3 Biotech},
	volume = {9},
	year = {2019},
	month = {JUN},
	pages = {237},
	type = {Article},
	abstract = {<p>Distiller-dried grain solid (DDGS), a co-product of alcohol production, contains cereal grain residues, proteins, and yeast metabolites, which make it suitable in poultry feeding. However, high phytate content of DDGS limits its applicability in poultry feed. In this study, Plackett-Burman design was used to improve cell-bound phytase production by Williopsis saturnus NCIM 3298, and we achieved an enzyme activity of 269IU/g of dry-wet biomass. The effect of this enhanced phytase-displaying yeast strain on hydrolysis of corn phytate and subsequently on ethanol production and DDGS quality was then investigated. Results of saccharification in the presence of phytase showed that reducing sugar content of liquefied mash increased by 11\%, which subsequently improved the ethanol production by 18\% (w/v) (p\&lt;0.01) compared with the control. Notably, phytase treatment decreased the phytate content of corn by 70\% (p\&lt;0.01) compared with the control, thereby improving the availability of free phosphate in fermentation broth and DDGS. Thus, the results obtained suggest that the addition of W. saturnus NCIM 3298 strain has the potential of providing a new source of phytase that would be useful in the feed and ethanol industries.</p>
},
	keywords = {DDGS, Ethanol, Phytase, Plackett-Burman Design, Williopsis saturnus NCIM 3298},
	issn = {2190-572X},
	doi = {10.1007/s13205-019-1764-y},
	author = {Pable, Anupama A. and Shah, Sarah and Kumar, V. Ravi and Khire, Jayant M.}
}
