@article { ISI:000301016800041,
	title = {Genetic homogeneity of in vitro raised plants of grapevine cv. crimson seedless revealed by ISSR and microsatellite markers},
	journal = {South African Journal of Botany},
	volume = {78},
	year = {2012},
	month = {JAN},
	pages = {302-306},
	publisher = {ELSEVIER SCIENCE BV},
	address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS},
	abstract = {<p>The present study was conducted to test the clonal homogeneity of six month old tissue culture raised plants of grapevine cv. Crimson Seedless using Inter Simple Sequence Repeat (ISSR) and Simple Sequence Repeat (SSR) markers. Visible assessment of these in vitro raised plants maintained in polyhouse did not show any morphological differences among themselves. However, to test the genetic homogeneity of these plants, we screened 50 ISSR primers out of which, 22 primers produced scorable and repeatable bands. These 22 primers were used further for assessing genetic homogeneity of in vitro raised plants of Crimson Seedless. These 22 ISSR primers generated 134 distinct band classes with a total of 3216 scorable bands. All the primers showed uniform banding pattern for all the in vitro raised plants and the mother plant. In case of 5 SSR primers (VS I, VVMD5, VVS2, VMCNG4c8 and VVMD31) used, a total of 288 scorable bands were obtained. The allele sizes ranged from 98 to 254 bp. Allelic composition of 23 in vitro raised plants and the mother plant at 5 SSR loci did not show any polymorphism. The results of the two marker systems in the present study revealed the genetic uniformity among the in vitro raised plants demonstrating the reliability of in vitro propagation system used for the cultivar. (C) 2011 SAAB. Published by Elsevier B.V. All rights reserved.</p>},
	keywords = {Allele composition, Clonal fidelity, Inter Simple Sequence Repeats, Monomorphic bands, Tissue culture, Vitis vinifera L.},
	issn = {0254-6299},
	doi = {10.1016/j.sajb.2011.08.009},
	author = {Nookaraju, A. and Agrawal, Dinesh C.}
}
@article {46638,
	title = {Generation of novelties in the genus Ocimum as a result of natural hybridization: a morphological, genetical and chemical appraisal},
	journal = {Industrial Crops and Products},
	volume = {156},
	year = {2020},
	month = {NOV},
	pages = {112859},
	type = {Article},
	abstract = {<p>The genus Ocimum is a boutique of a diverse set of specialized metabolites such as terpenoids and phenylpropanoids. Each Ocimum species and its cultivars represent a characteristic chemical profile. The present study explored the two interspecific Ocimum hybrids originating through a serendipitous natural cross between O. kilimandscharicum and O. basilicum. These two novel Ocimum hybrids exhibited intermediate morphological features of two parental species. Inter simple sequence repeats (ISSR) analysis and DNA barcoding with the plastid non-coding trnH-psbA intergenic spacer region reaffirmed unambiguous parental identification and differentiation of these natural hybrids from other available Ocimum species. Consequently, gas chromatographymass spectrometry-based metabolite profiling of two hybrids identified them as specific chemotypes with the presence of a unique blend of specialized metabolites from the parental species, which are either rich in terpenes or phenylpropanoids. Additionally, expression analysis of key genes from terpenoid and phenylpropanoid pathways corroborated with differential metabolite accumulation. Thus, these two Ocimum hybrids represented the novel chemotypes, which could be useful in commercial cultivation to produce novel essential oil and bioactive constituents. Further, the resulting metabolite diversity could have potential industrial applications in the areas of healthcare, bioremediation, and crop protection.</p>
},
	keywords = {Chemotype, DNA barcoding, Inter Simple Sequence Repeats, Interspecific hybrid, O. basilicum, O. kilimandscharicum, Ocimum},
	issn = {0926-6690},
	doi = {10.1016/j.indcrop.2020.112859},
	author = {Gurav, Tanuja P. and Jayaramaiah, Ramesha H. and Punekar, Sachin A. and Dholakia, Bhushan B. and Giri, Ashok P.}
}
