@article { ISI:000313009000014,
	title = {Over-expression of a subgroup 4 R2R3 type MYB transcription factor gene from Leucaena leucocephala reduces lignin content in transgenic tobacco},
	journal = {Plant Cell Reports},
	volume = {32},
	number = {1},
	year = {2013},
	month = {JAN},
	pages = {161-171},
	publisher = {SPRINGER},
	address = {233 SPRING ST, NEW YORK, NY 10013 USA},
	abstract = {<p>LlMYB1 , a subgroup 4 R2R3-type MYB transcription factor gene from Leucaena leucocephala appears to be a repressor of lignin biosynthesis pathway by regulating the transcription of general phenylpropanoid pathway genes. R2R3MYB transcription factors are known to play a wide role in regulating the phenylpropanoid pathway in plants. In this study, we report isolation, cloning and characterization of an R2R3MYB transcription factor gene (LlMYB1) from an economically important tree species, Leucaena leucocephala. LlMYB1 consists of 705 bp coding sequence corresponding to 235 amino acids. Sequence alignment revealed that the N-terminal (MYB) domain of the gene shares up to 95 \% similarity with subgroup 4 (Sg4) members of R2R3Myb gene family functionally known to be lignin repressors. Highly divergent C-terminal region of the gene carried an ERF-associated amphiphilic repression (EAR) motif, another characteristic of the Sg4. The gene was phylogenetically grouped closest with AmMYB308, a known repressor of monolignol biosynthetic pathway genes. Spatio-temporal expression studies at different ages of seedlings using quantitative real-time PCR (QRT-PCR) showed highest transcript level of the gene in 10 day old stem tissues. Over-expression of the gene in transgenic tobacco showed statistically significant decline in the transcript levels of the general phenylpropanoid pathway genes and reduction in lignin content. Our study suggests that LlMYB1 might be playing the role of a repressor of lignin biosynthesis in L. leucocephala.</p>},
	keywords = {Leucaena leucocephala, lignin, R2R3MYB, Repressor, Transcript level},
	issn = {0721-7714},
	doi = {10.1007/s00299-012-1350-9},
	author = {Omer, Sumita and Kumar, Santosh and Khan, Bashir Mohammad}
}
@article {49508,
	title = {MYB proteins: Versatile regulators of plant development, stress responses, and secondary metabolite biosynthetic pathways},
	journal = {International Journal of Biological Macromolecules},
	volume = {288},
	year = {2025},
	month = {FEB},
	pages = {138588},
	type = {Review},
	abstract = {<p>
	MYB proteins are ubiquitous in nature, regulating key aspects of plant growth and development. Although MYB proteins are known for regulating genes involved in secondary metabolite biosynthesis, particularly phenylpropanoids, their roles in terpenoid, glucosinolate, and alkaloid biosynthesis remain less understood. This review explores the structural and functional differences between activator and repressor MYB proteins along with their roles in plant growth, development, stress responses, and secondary metabolite production. MYB proteins serve as central hubs in protein-protein interaction networks that regulate expression of numerous genes involved in the adaptation of plants to varying environmental conditions. Thus, we also highlight key interacting partners of MYB proteins and their roles in these adaptation mechanisms. We further discuss the mechanisms regulating MYB proteins, including autoregulation, epigenetics, and post-transcriptional and post-translational modifications. Overall, we propose MYB proteins as versatile regulators for improving plant traits, stress responses, and secondary metabolite production.</p>
},
	keywords = {Activator, defense, Development, MYB protein, Repressor, Secondary metabolites},
	issn = {0141-8130},
	doi = {10.1016/j.ijbiomac.2024.138588},
	author = {Bhatt, Preshita A. and Gurav, Tanuja P. and Kondhare, Kirtikumar R. and Giri, Ashok P.}
}
