@article { ISI:000303055200006,
	title = {In vitro morphogenic response in cotyledon explants of semecarpus anacardium L.},
	journal = {Plant Biotechnology Reports},
	volume = {6},
	number = {2},
	year = {2012},
	month = {APR},
	pages = {141-148},
	publisher = {SPRINGER},
	address = {233 SPRING ST, NEW YORK, NY 10013 USA},
	abstract = {<p>Three different morphogenic responses-caulogenesis, direct somatic embryogenesis, and callusing-were noted in cotyledon explants of Semecarpus anacardium L. cultured in woody plant medium (WPM) containing thidiazuron (TDZ). Thidiazuron, at all concentrations tested, induced organogenic as well as embryogenic responses. The organogenic buds differentiated to shoots and the embryogenic mass (EM) gave rise to globular embryos which differentiated up to cotyledon-stage embryos on repeated culture in growth regulator (GR)-free WPM medium containing 0.2\% activated charcoal after the removal of TDZ. The organogenic and embryogenic responses were optimal in 9.08 mu M TDZ after the removal of TDZ. Elongated shoots rooted in half-strength liquid WPM medium with 2.46 mu M indole butyric acid. Plants were successfully acclimatized and transferred to soil. Histological studies confirmed the direct origin of the organogenic buds from the cotyledon explants. The EMs produced somatic embryos on repeated culture in charcoal incorporated GR-free medium. Morphogenic callus formation from the cotyledon explants was also noted. This callus on repeated culture in WPM medium with charcoal differentiated into somatic embryos. Repetitive somatic embryogenesis was evident from direct and indirectly formed primary embryos. The somatic embryos did not convert into plantlets, though sporadic germination of embryos was observed through the emergence of roots.</p>},
	keywords = {callus, Caulogenesis, Globular embryos, Growth regulator, Histology, somatic embryogenesis},
	issn = {1863-5466},
	doi = {10.1007/s11816-011-0207-y},
	author = {Panda, Bhuban Mohan and Hazra, Sulekha}
}
@article { ISI:000317836000016,
	title = {Extra- and intracellular gold nanoparticles synthesis using live peanut callus cells},
	journal = {Current Nanoscience},
	volume = {9},
	number = {1},
	year = {2013},
	month = {FEB},
	pages = {107-112},
	publisher = {BENTHAM SCIENCE PUBL LTD},
	address = {EXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES},
	abstract = {<p>An important challenge of considerable topical significance in nanotechnology is the development of eco-friendly experimental processes for the synthesis of nanomaterials in large quantities with variable sizes, shapes and chemical compositions. Green synthesis routes for the production of inorganic metal nanoparticles using whole cell of microorganisms and plant extracts are gaining tremendous popularity as these are non-toxic, cheap and occur at ambient conditions. The present work emphasizes on gold nanoparticles synthesis protocol using live plant callus cells. Peanut callus cells when incubated with HAuCl4 solution in ambient conditions reduced the precursor and lead to formation of well dispersed, water soluble extracellular and intracellular gold nanoparticles within 24 hours. The biosynthesis of gold nanoparticles was monitored by UV-visible spectroscopy (UV-Vis) and further characterized by X-ray diffraction analysis (XRD), Energy Dispersive Spectroscopy (EDS) and Selected Area Electron Diffraction (SAED). The particle size distribution shows that the average particle size is 50 nm for extra-and 31 nm for intracellular gold nanoparticles. The nanoparticles may be stabilized by proteins secreted by callus cells. The reduction process is believed to occur enzymatically, thus creating the possibility of a rational, plant cell-based method for the synthesis of nanoparticles over a wide range of chemical compositions.</p>},
	keywords = {Biosynthesis, callus, gold nanoparticles, live cells, Peanut, TEM},
	issn = {1573-4137},
	author = {Raju, D. and Mehta, Urmil J. and Ahmad, Absar}
}
