@article { ISI:000315557700001,
	title = {Isolation, fractionation and evaluation of the antiplasmodial properties of Phyllanthus niruri resident in its chloroform fraction},
	journal = {Asian Pacific Journal of Tropical Medicine},
	volume = {6},
	number = {3},
	year = {2013},
	month = {MAR},
	pages = {169-175},
	publisher = {ELSEVIER SCI LTD},
	address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND},
	abstract = {<p>Objective: To investigate the antiplasmodial activity of Phyllanthus niruri (P. niruri) methanol extract (ME) and its fractions in mice. Methods: P. niruri methanol extract and its chloroform, ethanol and aqueous portions were tested against chloroquine-sensitive Plasmodium berghei berghei. in early, established mid repository models of infection using Knight and Peter{\textquoteright}s 4-day suppressive model, Ryley and Peters curative model and Peters prophylactic model respectively. Results: Chemosuppression of parasitaemia (37.65\%-50.53 \%) was elicited by 100-400 mg/kg (b.w.) of ME. At doses of 100 mg/kg b.w., the chloroform fraction (F1) significantly (P\&lt;0.01) suppressed parasitaemia by 85.29\%, while ethanol and aqueous fractions (F2 and F3, respectively) elicited 67.06\% and 51.18\% chemosuppression. The most active fraction, F1 was selected for further antiplasmodial screening. In established infection, ME reduced parasitaemia (15.81\%-62.96\%) while F1 significantly (P\&lt;0.01) reduced parasitaemia (44.36\%-90.48\%), with effects comparable to that of chloroquine (96.48\%). The prophylactic antiplasmodial activity of ME (92.50\% suppression) was also significant (P\&lt;0.01) and was more effective than pyrimethamine (85.00\%). Additionally, cell membrane integrity of non-parasitized erythrocytes incubated with 125-500 mg/mL F1 was maintained. Conclusions: These findings indicate the antiplasmodial efficacy of P. niruri methanol extract, and the localization of this effect in its chloroform fraction.</p>},
	keywords = {malaria, Phyllanthus niruri, Plasmodium},
	issn = {1995-7645},
	doi = {10.1016/S1995-7645(13)60018-8},
	author = {Ifeoma, Obidike and Samuel, Okhale and Itohan, Aboh Mercy and Adeola, Salawu Oluwakanyinsola}
}
