@article { ISI:000243492800021, title = {Enantiomeric separation of novel anticancer agent 5-hydroxy-3-(4-methoxyphenyl)-2-(3,4,5-trimethoxyphenyl)-cyclopent-2-en- 1-one}, journal = {Journal of Chromatography A}, volume = {1138}, number = {1-2}, year = {2007}, month = {JAN}, pages = {184-189}, publisher = {ELSEVIER SCIENCE BV}, type = {Article}, address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS}, abstract = {

The enantiomers of 5-hydroxy-3-(4-methoxyphenyl)-2-(3,4,5-trimethoxyphenyl)-cyclopent-2-en- 1-one, a novel anticancer agent, were separated by derivatisation with caronaldehyde, separation of the resulting diastereoisomers of the corresponding esters by silica gel column chromatography and regeneration of alcohols (S)-5-hydroxy-3-(4-methoxyphenyl)-2-(3,4,5-trimethoxyphenyl)-cyclopent-2 -en-1-one and (R)5-hydroxy-3-(4-methoxyphenyl)-2-(3,4,5-trimethoxyphenyl)-cyclopent-2- en- 1-one under aqueous conditions. The absolute configuration of the enantiomers was determined by H-1 NMR studies of the corresponding Mosher esters. Alternatively, the enantiomers were separated by preparative HPLC to collect the (S)- and (R)-5-hydroxy-3-(4-methoxyphenyl)-2-(3,4,5-trimethoxyphenyl)-cyclopent-2 -en-1-ones with high purity which was comparable with that obtained by the chemical method. The details of these methods have been presented herein. (c) 2006 Elsevier B.V. All rights reserved.

}, keywords = {Anticancer, column chromatography, diaryl cyclopentenone, diastereoisomers, enantioseparation, HPLC, Resolution}, issn = {0021-9673}, doi = {10.1016/j.chroma.2006.10.076}, author = {Shinde, Popat D. and Jadhav, Vinod H. and Borate, Hanumant B. and Bhide, Sunil R. and Sonawane, Kiran B. and Wakharkar, Radhika D.} } @article { ISI:000267619700020, title = {Influence of hormones and medium components on expression of dipyranocoumarins in cell suspension cultures of calophyllum inophyllum L.}, journal = {Process Biochemistry}, volume = {44}, number = {8}, year = {2009}, month = {AUG}, pages = {916-922}, publisher = {ELSEVIER SCI LTD}, address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND}, abstract = {

Cell suspension cultures were initiated separately from leaf and nodal/internodal calluses for the study of influence of hormones and medium components on biomass growth and expression of dipyranocoumarins. Highest 6.2 times biomass was enhanced in suspension cultures of nodal/internodal callus supplemented with threefold total sulphate. Picloram 8.28 mu M along with BAP 8.88 mu M enhanced 295.05 times inophyllum A in suspension cultures of leaf callus whereas IBA 14.70 mu M along with BAP 4.44 mu M in suspension cultures of leaf callus enhanced 1065 times inophyllum B. IBA 4.90 mu M alone in suspension cultures of nodal/internodal callus enhanced maximum 616 times inophyllum C. Only IBA 9.80 mu M in suspension cultures of leaf callus enhanced 23.22 times inophyllum P. Variation in nitrate and sulphate had maximum positive influence on expression of inophyllums A and C and vitamins had maximum positive influence on expression of inophyllums A, C and B. (C) 2009 Elsevier Ltd. All rights reserved.

}, keywords = {Calophyllum inophyllum, dipyranocoumarins, Expression pattern, HPLC, Medium components, Suspension culture}, issn = {1359-5113}, doi = {10.1016/j.procbio.2009.03.005}, author = {Pawar, Kiran D. and Thengane, Shubhada Ratnakar} } @article { ISI:000270759600015, title = {LC-UV and LC-MS evaluation of stress degradation behaviour of tenatoprazole}, journal = {Journal of Pharmaceutical and Biomedical Analysis}, volume = {50}, number = {5}, year = {2009}, month = {DEC}, pages = {787-793}, publisher = {PERGAMON-ELSEVIER SCIENCE LTD}, address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND}, abstract = {

In the present study, comprehensive stress testing of tenatoprazole was carried out according to ICH guideline Q1A (R2). Tenatoprazole was subjected to stress conditions of hydrolysis, oxidation, photolysis and neutral decomposition. Additionally, the solid drug was subjected to 50 degrees C for 60 days in dry-bath, and to the combined effect of temperature and humidity at 40 degrees C/75\% RH. Extensive degradation was found to occur in acidic, neutral and oxidative conditions. Mild degradation was observed in basic conditions. The drug is relatively stable in the solid-state. The products formed under different stress conditions were investigated by LC and LC-MS. Successful separation of drug from degradation products formed under stress conditions was achieved on a Chemito ODS-3 column [C(18) (5 mu m, 250 mm x 4.6 mm, i.d.)] using methanol: 0.01 M acetate buffer pH 4.5 adjusted with glacial acetic acid (55:45) as the mobile phase at flow rate of 1 mL/min and the peak was detected using a UV detector set at 306 nm. The LC-MS m/z values and fragmentation patterns of degradation products formed under different stress conditions were studied and characterized through LC-MS fragmentation. Based on the results, degradation pathway for drug has been proposed. (C) 2009 Elsevier B.V. All rights reserved.

}, keywords = {HPLC, Stability-indicating method, Stress studies, Tenatoprazole, Validation}, issn = {0731-7085}, doi = {10.1016/j.jpba.2009.06.026}, author = {Mahadik, Mahadeo V. and Bhusari, Vidhya K. and Kulkarni, Mahesh J. and Dhaneshwar, Sunil R.} } @article { ISI:000288661500011, title = {Association between chemical and genetic variation in calophyllum inophyllum, a medicinally important tree of the Western Ghats of India}, journal = {Plant Systematics and Evolution}, volume = {292}, number = {3-4}, year = {2011}, month = {APR}, pages = {257-265}, publisher = {SPRINGER WIEN}, address = {SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA}, abstract = {

The objective of the present work is to study the chemical variation in Calophyllum inophyllum growing along the Western Ghats of India. Contents of dipyranocoumarins (inophyllums) in C. inophyllum were determined to assess whether they could be used as a taxonomic marker for C. inophyllum. This study also aims to establish inter simple sequence repeat (ISSR) markers that can be used to study genetic variation within the species and explore correlation between ISSR and chemical markers. The contents of dipyranocoumarins were estimated in seeds collected from 20 locations. Leaves from plants at the same 20 locations were assayed for ISSR variation. A dendrogram based on Nei{\textquoteright}s genetic distance as well as principal component analysis based on dipyranocoumarins and ISSR variation clustered plants from these 20 locations into three groups that indicated close relationship among ISSR, dipyranocoumarins contents, and geographical position (variation) of locations. Based on this study, two locations of elite plants were identified.

}, keywords = {Calophyllum inophyllum, Dendrogram, dipyranocoumarins, HPLC, ISSR, Principal component analysis, Western Ghats of India}, issn = {0378-2697}, doi = {10.1007/s00606-010-0409-8}, author = {Pawar, Kiran D. and Joshi, Swati P. and Thengane, Shubhada Ratnakar} } @article { ISI:000301295900015, title = {Structural determination and chemical esterification of the sophorolipids produced by Candida bombicola grown on glucose and alpha-linolenic acid}, journal = {Biotechnology Letters}, volume = {34}, number = {4}, year = {2012}, month = {APR}, pages = {701-707}, publisher = {SPRINGER}, address = {VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS}, abstract = {

The extracellular surface-active glycolipids produced by the yeast, Candida bombicola when grown on glucose and alpha-linolenic acid, were analyzed by HPLC with electro-spray ionization (ESI-MS) and collision-induced dissociation mass spectrometry. The analysis confirmed that the sophorolipid (SL) mixture contained three different forms of C18:3 SL molecules: free acid, lactone and a diacetylated lactone, which has not been reported previously. Also a minor amount of diacetylated lactone form of C18:1 SL was detected. Further, the SL mixture was subjected to chemical esterification reaction with sodium methoxide. The reaction product was analyzed with ESI-MS and confirmed to be the single homogenous esterified product containing C18:3 moieties in its fatty acid chain.

}, keywords = {Candida bombicola, Collision-induced dissociation mass spectrometry, Electrospray ionization mass spectrometry, HPLC, Linolenic acid, Sophorolipid methyl ester}, issn = {0141-5492}, doi = {10.1007/s10529-011-0818-y}, author = {Gupta, Reetika and Prabhune, Asmita} } @article { ISI:000325642800010, title = {Production, purification and characterization of cholesterol oxidase from a newly isolated streptomyces sp.}, journal = {World Journal of Microbiology \& Biotechnology}, volume = {29}, number = {11}, year = {2013}, month = {NOV}, pages = {2077-2085}, publisher = {SPRINGER}, address = {233 SPRING ST, NEW YORK, NY 10013 USA}, abstract = {

Cholesterol oxidase production (COD) by a new isolate characterized as Streptomyces sp. was studied in different production media and fermentation conditions. Individual supplementation of 1 \% maltose, lactose, sucrose, peptone, soybean meal and yeast extract enhanced COD production by 80-110 \% in comparison to the basal production medium (2.4 U/ml). Supplementation of 0.05 \% cholesterol (inducer) enhanced COD production by 150 \%. COD was purified 14.3-fold and its molecular weight was found to be 62 kDa. V-max (21.93 mu M/min mg) and substrate affinity K-m (101.3 mu M) suggested high affinity of the COD for cholesterol. In presence of Ba2+ and Hg2+ the enzyme activity was inhibited while Cu2+ enhanced the activity nearly threefold. Relative activity of the enzyme was found maximum in triton X-100 whereas sodium dodecyl sulfate inactivated the enzyme. The enzyme activity was also inhibited by the thiol-reducing reagents like Dithiothreitol and beta-mercaptoethanol. The COD showed moderate stability towards all organic solvents except acetone, benzene and chloroform. The activity increased in presence of isopropanol and ethanol. The enzyme was most active at pH 7 and 37 A degrees C temperature. This organism is not reported to produce COD.

}, keywords = {Affinity chromatography, Cholesterol oxidase, HPLC, SDS PAGE, Streptomyces sp.}, issn = {0959-3993}, doi = {10.1007/s11274-013-1371-8}, author = {Niwas, Ram and Singh, Vineeta and Singh, Rajbir and Tripathi, Divya and Tripathi, C. K. M.} }