@article { ISI:000380574100066,
	title = {Azadirachta indica triterpenoids promote osteoblast differentiation and mineralization in vitro and in vivo},
	journal = {Bioorganic \& Medicinal Chemistry Letters},
	volume = {26},
	number = {15},
	year = {2016},
	month = {AUG},
	pages = {3719-3724},
	publisher = {PERGAMON-ELSEVIER SCIENCE LTD},
	address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND},
	abstract = {<p>Terpenoids were isolated using chromatographic purification through solvent purification technique and identified as Azadirone (1), Epoxyazadiradione (2) Azadiradione (3) Gedunin (4) Nimbin (5) Salannin (6) Azadirachtin A (7) and Azadirachtin B (8) from Azadirachta indica. Out of eight compounds, only three compounds had osteogenic activity and enhanced osteoblast proliferation, differentiation and mineralization in osteoblast cells. Active compounds stimulated osteogenic genes ALP, RunX-2 and OCN expressions in vitro, but Azadirachtin A had a maximum ability to stimulate osteoblast differentiation and mineralization compared to other two active compounds. For in vivo study, Azadirachtin A injected subcutaneously in pups, which enhanced osteogenic gene expressions and promoted bone formation rate significantly. Here, we conclude that active compounds of Azadirachta indica have osteogenic activity and Azadirachtin A has a beneficial effects on bone. (C) 2016 Elsevier Ltd. All rights reserved.</p>},
	keywords = {ALP and mineralization assay, Balb/c mice pups, Calvarial osteoblast cells, Gene expressions},
	issn = {0960-894X},
	doi = {10.1016/j.bmcl.2016.05.076},
	author = {Kushwaha, Priyanka and Khedgikar, Vikram and Haldar, Saikat and Gautam, Jyoti and Mulani, Fayaj A. and Thulasiram, Hirekodathakallu V. and Trivedi, Ritu}
}
