@article { ISI:000361828000068, title = {Conversion of dried aspergillus candidus mycelia grown on waste whey to biodiesel by in situ acid transesterification}, journal = {Bioresource Technology}, volume = {197}, year = {2015}, month = {DEC}, pages = {502-507}, publisher = {ELSEVIER SCI LTD}, address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND}, abstract = {

This study reports optimization of the transesterification reaction step on dried biomass of an oleaginous fungus Aspergillus candidus grown on agro-dairy waste, whey. Acid catalyzed transesterification was performed and variables affecting esterification, viz., catalyst methanol and chloroform concentrations, temperature, time, and biomass were investigated. Statistical optimization of the transesterification reaction using Plackett-Burman Design showed biomass to be the predominant factor with a 12.5-fold increase in total FAME from 25.6 to 320 mg. Studies indicate that the transesterification efficiency in terms of conversion is favored by employing lower biomass loadings. A. candidus exhibited FAME profiles containing desirable saturated (30.2\%), monounsaturated (31.5\%) and polyunsaturated methyl esters (38.3\%). The predicted and experimentally determined biodiesel properties (density, kinematic viscosity, iodine value, cetane number, TAN, water content, total and free glycerol) were in accordance with international (ASTM D6751, EN 14214) and national (IS 15607) standards. (C) 2015 Published by Elsevier Ltd.

}, keywords = {Aspergillus candidus, Biodiesel, In situ acid transesterification, Plackett-Burman Design, Whey}, issn = {0960-8524}, doi = {10.1016/j.biortech.2015.07.118}, author = {Kakkad, Hardik and Khot, Mahesh and Smita S. Zinjarde and RaviKumar, Ameeta and Kumar, V. Ravi and Kulkarni, B. D.} } @article {44228, title = {Optimization of the in situ transesterification step for biodiesel production using biomass of Yarrowia lipolytica NCIM 3589 grown on waste cooking oil}, journal = {Energy}, volume = {142}, year = {2018}, month = {JAN }, pages = {944-952}, type = {Article}, abstract = {

The in situ (one-step) acid-catalyzed transesterification step for conversion to biodiesel of biomass from oleaginous yeast Yarrowia lipolytica grown on waste cooking oil (WCO) is studied. The process yield of biodiesel was optimized by investigating effects of various parameters, namely, biomass, methanol, chloroform, catalyst, temperature, time and sonication. A Plackett-Burman statistical design of experiments revealed that biomass is the most significant factor influencing biodiesel (FAME, fatty acid methyl ester) production. Subsequently, a one variable design (OVD) of experiments for increased biomass loadings showed higher yields of FAME with no additional requirement of reactants, solvents or special equipment. The biomass grown on WCO had a lipid productivity of 0.042 g L-1 h(-1) and 4 g of this loading gave a high FAME yield of 0.88 gin 8 hat 50 degrees C with methanol: chloroform (10:1) and acid catalyst (0.2 M H2SO4,1.0 ml g(-1)). The FAME profile had desirable amounts of saturated (32.81\%), monounsaturated (36.41\%), polyunsaturated (30.59\%) methyl esters. The predicted and experimentally determined physico-chemical properties of FAME were found in accordance with specified international standards. Thus, the direct one-pot in situ transesterification reaction using Y. lipolytica biomass grown on WCO provides a high yield of biodiesel with potential applicability while simultaneously addressing the management of this pollutant. (C) 2017 Elsevier Ltd. All rights reserved.

}, keywords = {Biodiesel, FAME, In situ transesterification, One -step, Waste cooking oil, Yarrowia lipolytica}, issn = {0360-5442}, doi = {10.1016/j.energy.2017.10.082}, author = {Katre, Gouri and Raskar, Shubham and Zinjarde, Smita and Kumar, V. Ravi and Kulkarni, B. D. and RaviKumar, Ameeta} } @article {49541, title = {Aspergillus terreus variant TB21 wet biomass optimized by in-situ transesterification for biodiesel production}, journal = {AMB Express}, volume = {15}, year = {2025}, month = {FEB}, pages = {23}, type = {Article}, abstract = {

The oleaginous fungus, Aspergillus terreus when subjected to random chemical mutagenesis led to isolation of TB21 variant with improved lipid content (78.1\%) as compared to wild type (49.8\%). The fungal wet biomass grown on sugarcane bagasse hydrolysate (SCBH) was subjected to one-step in-situ (direct) acid transesterification to optimize its conversion to biodiesel using a 2-level factorial statistical design of experiments. The process optimization revealed that wet biomass and methanol were the most significant factors and in a short reaction time period of 5 min with low methanol: wet biomass ratio (10:1) influenced FAME production Statistical optimization studies showed that TB21 exhibited a higher FAME content of 76.5 and 38.1\% (w/w) from wet and dry biomass, respectively when compared to wild type (48.1 and 24.5\%). FAME productivity (0.55-1 h-1) and a yield (66 gL-1) were achieved when TB21 was grown on SCBH for 120 h at 30 degrees C. The FAME profile from the wet biomass of TB21 grown on SCBH had desirable amounts of saturated (77.7\%), monounsaturated (7.2\%), and polyunsaturated (2.4\%) methyl esters. Physico-chemical properties of TB21-derived biodiesel were determined, namely, density(0.88 g cm-3), kinematic viscosity (4.1 mm s-2), iodine value (96.82), cetane number (55.31), free fatty acid content (0.15\%), total acid number (0.3 NaOH mg g-1), meeting international (ASTM D6751, EN 14214) and Indian (IS 15607) standards. Thus, the direct one-pot in situ transesterification reaction using wet biomass of variant TB21 strain showed improved production and quality of biodiesel with potential large scale application using the low-cost substrate (SCBH).

}, keywords = {Aspergillus Terreus, Biodiesel, In situ transesterification, Mutant, Statistical optimization, Wet biomass}, issn = {2191-0855}, doi = {10.1186/s13568-024-01772-7}, author = {Bed, Rashmi K. and Kumar, V. Ravi and RaviKumar, Ameeta} }