@article {44228,
	title = {Optimization of the in situ transesterification step for biodiesel production using biomass of Yarrowia lipolytica NCIM 3589 grown on waste cooking oil},
	journal = {Energy},
	volume = {142},
	year = {2018},
	month = {JAN },
	pages = {944-952},
	type = {Article},
	abstract = {<p>The in situ (one-step) acid-catalyzed transesterification step for conversion to biodiesel of biomass from oleaginous yeast Yarrowia lipolytica grown on waste cooking oil (WCO) is studied. The process yield of biodiesel was optimized by investigating effects of various parameters, namely, biomass, methanol, chloroform, catalyst, temperature, time and sonication. A Plackett-Burman statistical design of experiments revealed that biomass is the most significant factor influencing biodiesel (FAME, fatty acid methyl ester) production. Subsequently, a one variable design (OVD) of experiments for increased biomass loadings showed higher yields of FAME with no additional requirement of reactants, solvents or special equipment. The biomass grown on WCO had a lipid productivity of 0.042 g L-1 h(-1) and 4 g of this loading gave a high FAME yield of 0.88 gin 8 hat 50 degrees C with methanol: chloroform (10:1) and acid catalyst (0.2 M H2SO4,1.0 ml g(-1)). The FAME profile had desirable amounts of saturated (32.81\%), monounsaturated (36.41\%), polyunsaturated (30.59\%) methyl esters. The predicted and experimentally determined physico-chemical properties of FAME were found in accordance with specified international standards. Thus, the direct one-pot in situ transesterification reaction using Y. lipolytica biomass grown on WCO provides a high yield of biodiesel with potential applicability while simultaneously addressing the management of this pollutant. (C) 2017 Elsevier Ltd. All rights reserved.</p>},
	keywords = {Biodiesel, FAME, In situ transesterification, One -step, Waste cooking oil, Yarrowia lipolytica},
	issn = {0360-5442},
	doi = {10.1016/j.energy.2017.10.082},
	author = {Katre, Gouri and Raskar, Shubham and Zinjarde, Smita and Kumar, V. Ravi and Kulkarni, B. D. and RaviKumar, Ameeta}
}
@article {48792,
	title = {Potential of Y. lipolytica epoxide hydrolase for efficient production of enantiopure (R)-1,2-octanediol},
	journal = {AMB Express},
	volume = {13},
	year = {2023},
	month = {JUL },
	pages = {77},
	type = {Article},
	abstract = {<p>
	The recombinant Yleh from a tropical marine yeast Yarrowia lipolytica NCIM 3589 exhibited a high epoxide hydrolase activity of 9.34 +/- 1.80 mu mol min(-1) mg(-1) protein towards 1,2-epoxyoctane (EO), at pH 8.0 and 30 degrees C. The reaction product was identified as 1,2-Octanediol (OD) by GC-MS using EO and H2O18 as substrate, affirming the functionality of Yleh as an epoxide hydrolase. For EO, the K-m, V-max, and k(cat)/K-m values were 0.43 +/- 0.017 mM, 0.042 +/- 0.003 mM min(-1), and 467.17 +/- 39.43 mM(-1) min(-1), respectively. To optimize the reaction conditions for conversion of racemic EO by Yleh catalyst to enantiopure (R)-1,2-octanediol, initially, Response Surface Methodology was employed. Under optimized reaction conditions of 15 mM EO, 150 mu g purified Yleh at 30 degrees C a maximal diol production of 7.11 mM was attained in a short span of 65 min with a yield of 47.4\%. Green technology using deep eutectic solvents for the hydrophobic substrate (EO) were tested as co-solvents in Yleh catalyzed EO hydrolysis. Choline chloride-Glycerol, produced 9.08 mM OD with an increased OD yield of 60.5\%. Thus, results showed that deep eutectic solvents could be a promising solvent for Yleh-catalyzed reactions making Yleh a potential biocatalyst for the biosynthesis of enantiopure synthons. [GRAPHICS] .</p>
},
	keywords = {Deep Eutectic solvents, Optimization, Recombinant epoxide hydrolase, Response surface methodology, Yarrowia lipolytica},
	issn = {2191-0855},
	doi = {10.1186/s13568-023-01584-1},
	author = {Godase, Vijaya P. P. and Kumar, V. Ravi and Kumar, Ameeta Ravi}
}
